Beschrijving
Product Information
|
Product Name |
Cat No. |
Specification |
|
Recombinant Human NKG2A&CD94 Protein,mFc Tag |
94977ES25 |
25 μg |
|
94977ES60 |
100 μg |
|
|
94977ES76 |
500 μg |
Product Properties
|
Synonyms |
CD94; CD159a; NKG2-A; |
|
Source |
Human NKG2A&CD94 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus. It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94).[Accession | P26715-1(NKG2A)&Q13241-1(CD94)] |
|
Molecular Weight |
The protein has a predicted MW of 56.9 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on SDS-PAGE result. |
|
Endotoxin |
< 1EU per μg by the LAL method. |
|
Activity |
Blocking Data: Serial dilutions of Anti-NKG2A Antibody were added into Biotinylated Human HLA-E*01:03 Complex Tetramer, His Tag : Human NKG2A&CD94, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.15μg/ml. ELISA Data: Immobilized Human NKG2A&CD94, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA. SPR Data: Human NKG2A&CD94, mFc Tag captured on CM5 Chip via Anti-mouse Antibody can bind Human HLA-E Complex Tetramer, His Tag with an affinity constant of 7.90 nM as determined in SPR assay . |
|
Purity |
> 95% as determined by SDS-PAGE and by HPLC. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3-6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1. Please operate with lab coats and disposable gloves,for your safety.
2. This product is for research use only.
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