Beschrijving
Product properties
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Synonyms |
ATF; Urokinase;PLAU; URK |
|
Source |
Cynomolgus PLAU/uPA Protein (active form) is expressed from HEK293 with His tag at the C-Terminus. It contains Ser21-Leu430, which consists of two chains: Long chain A and chain B. The long chain A is further cleaved to yield a short chain A and N-Terminus fragment.[Accession | A0A2K5WND1] |
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Molecular Weight |
The protein has a predicted MW of 47.23 kDa. Due to protein lysis and glycosylation, the protein migrates to 23-25 kDa (long chain A), 35-40 kDa (chain B) and 50-60 kDa (long chain A&chain B) based on SDS-PAGE result. |
|
Endotoxin |
Less than 1EU per μg by the LAL method. |
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Purity |
> 95% as determined by SDS-PAGE and HPLC. |
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Activity |
ELISA Data: Immobilized Cynomolgus PLAU, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 25.1ng/ml determined by ELISA. |
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Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
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