Beschrijving
Product properties
|
Synonyms |
NAG-1; MIC-1;GDF-15; PLAB |
|
Source |
Rat GDF15 Protein is expressed from E.coli with His tag at the N-Terminus. It contains Ser189-Ala303.[Accession | Q9Z0J6] |
|
Molecular Weight |
The protein has a predicted MW of 13.68 kDa. The protein migrates to 15-17 kDa based on SDS-PAGE result. |
|
Endotoxin |
Less than 1EU per μg by the LAL method. |
|
Purity |
> 95% as determined by SDS-PAGE |
|
Activity |
ELISA Data: Immobilized Rat GDF-15, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA .Immobilized Rat GDF15, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.95μg/ml determined by ELISA. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9). |
Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
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