Beschrijving
Specifications
|
Synonyms |
HGF R; oncogene MET;MET; AUTS9 |
|
Source |
Human HGF R/c-MET Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu25-Thr932.[Accession | P08581-1] |
|
Molecular Weight |
The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 72.1 kDa (β chain). Due to glycosylation, the protein migrates to 45-60 kDa (α subunit) and 80-100 kDa (β subunit) based on SDS-PAGE result. |
|
Endotoxin |
Less than 1EU per μg by the LAL method. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Activity |
ELISA Data: Immobilized Human HGF R, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA .Immobilized Human HGF R, His Tag at 2μg/ml (100μl/Well). Dose response curve for Human HGF, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage
The product should be stored at -25~-15℃ for 1 year from the date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves for your safety.
2.This product is for research use only.
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