Beschrijving
ICAM-1, intercellular adhesion molecule-1, which is constitutively present on endothelial cells, is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues.
Specifications
|
Synonyms |
ICAM-1; BB2; CD54 |
|
Uniprot No. |
AAH15969 |
|
Source |
Recombinant Human ICAM-1/CD54 Protein is expressed from HEK293 cells with His tag at the C-terminal. It contains Gln28-Glu480. |
|
Molecular Weight |
The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
<1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge tubes before opening. Dissolve lyophilized protein with sterile water to ensure the concentration is greater than 100 µg/mL. |
Storage
The product should be stored at -25~-15℃ for 1 year from the date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves for your safety.
2.This product is for research use only.
Product Data
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