Beschrijving
Specifications
|
Source |
Human SPARC Protein is expressed from HEK293 with His tag at the C-terminal. It contains Ala18-Ile303.[Accession | P09486] |
|
Molecular Weight |
The protein has a predicted MW of 33.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
|
Endotoxin |
Less than 1EU per μg by the LAL method. |
|
Purity |
> 95% as determined by Tris-Bis PAGE and HPLC |
|
活性（Activity） |
ELISA Data: Immobilized Human SPARC, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 0.10μg/ml determined by ELISA. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage
The product should be stored at -25~-15℃ for 1 year from the date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend aliquoting the protein into smaller quantities when first used and avoiding repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves the for your safety.
2.This product is for research use only.
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FAQ
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