Beschrijving
Specifications
Source |
Human PAI-1 Protein is expressed from HEK293 with His tag at the C-terminal. It contains Val24-Pro402.[Accession | P05121-1] |
Molecular Weight |
The protein has a predicted MW of 43.86 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Tris-Bis PAGE result. |
Source |
Less than 1EU per μg by the LAL method. |
Purity |
> 95% as determined by Tris-Bis PAGE and HPLC |
Formulation |
Lyophilized from 0.22 μm filtered solution in 50mM NaAC, 0.1M NaCl, 100mM L-arginine (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization. |
Appearance |
冻干粉 |
Storage
Store at -25~-15℃, valid for 1 year after receipt. After reconstitution, store at 2~8°C, valid for 7 days. After reconstitution, store at -85~-65℃, valid for 3 months.
Reconstitution
Centrifuge before opening. Dissolve the lyophilized protein in 50mM NaAC, 0.1M NaCl, 100mM L-arginine (pH 5.5). Reconstitution concentration exceeds 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.Note
Note
1.Please operate with lab coats and disposable gloves for your safety.
2.This product is for research use only.
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FAQ
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