Beschrijving
FCAR, also called Fc alpha RI or CD89, is a variably glycosylated 50-100 kDa myeloid-specific type I transmembrane (TM) Fc receptor for IgA that is a member of the multichain immune recognition receptor (MIRR) family. Shedding and circulation of polymeric IgA/FCAR immune complexes has been reported. Circulating neutrophils, eosinophils, and monocytes express FCAR. Tissue expression of FCAR is mainly from neutrophils; FCAR is downregulated as monocytes differentiate to tissue macrophages. On neutrophils, a significant amount of FCAR lacks FcR gamma, but can still be endocytosed to early endosomes and recycled to the cell surface. Binding of serum IgA to FCAR is transient and anti-inflammatory, inhibiting IgG or IgE-induced degranulation. Sustained aggregation of FCAR results in inflammatory responses. FcR gamma signaling is required for these and for transport to late endosomes. FCAR structure resembles the KIR/ILT/LIR/MIR family more than other IgA receptors, including pIgR, Fc alpha /μR, asialoglycoprotein receptor (ASGR1) and transferrin receptor (TfR).
Specifications
|
Synonyms |
IgA Fc receptor; CD89; FCAR; CTB-61M7.2; FcalphaRI; Fc fragment of IgA |
|
Uniprot No. |
Q9UIB8.1 |
|
Source |
Recombinant Human CD89 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Gln22-Asn227. |
|
Molecular Weight |
Approximately 24.6 kDa. Due to glycosylation, the protein migrates to 45-65 kDa based on Tris-Bis PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Source |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
preparation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Storage
Transported with ice packs. Store at -20℃ to -80℃, valid for one year.
After reconstitution, store at -20 to -80°C in unopened state for 3-6 months. After reconstitution, store at 2-8°C for 2-7 days.
It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing.
Note
1. Avoid repeated freezing and thawing.
2. For your safety and health, please wear a lab coat and disposable gloves when operating.
3. This product is for scientific research purposes only.
Product Data
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