Beschrijving
|
Product Alias |
PLAU, Urokinase, ATF, UPA, URK, u-PA, BDPLT5, QPD |
|
Species |
Human |
|
Expression sequence |
Ser21-Leu431 |
|
Accession |
P00749-1 |
|
Label |
C-His |
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Expression system |
HEK293 |
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Molecular weight |
The protein has a predicted MW of 47.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
preparation |
Lyophilized from 0.22μm filtered solution in 25mM HEPES, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
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Appearance |
Lyophilized Powder |
|
Biological Activity |
【ELISA Data】Immobilized Human PLAU, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. |
|
Reconstitution method |
Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing. |
Product Features
High purity, high activity, low endotoxin, high batch consistency.
Storage
Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.
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