Beschrijving
Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100.
Specifications
|
Nature name |
Nature Details |
|
Product Alias |
HLA0201, MHC I, gp100, Glycoprotein 100, ME20-M, P100 |
|
Species |
Human |
|
Expression sequence |
Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and YLEPGPVTA peptide |
|
Accession |
A0A140T913 (HLA-A*02:01)&P61769 (B2M)&YLEPGPVTA |
|
Label |
C-His-Avi |
|
Expression system |
HEK293 |
|
Molecular weight |
Approximately 50.4 kDa.Due to glycosylation, the protein migrates to52-62 kDa based on Tris-Bis PAGE result. |
|
purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
preparation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
【ELISA Data】Immobilized Human GP100 (HLA-A*02:01) Complex, His Tag at 2μg/mL(100μL/Well) on the plate. Dose response curve for Anti-GP100 (HLA-A*02:01) Antibody, hFc Tag with the EC50of 32.9ng/mLdetermined by ELISA. |
|
Reconstitution method |
Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.。 |
Product Features
High purity, high activity, low endotoxin, high batch consistency
Application Cases
Product Data
Tris-Bis PAGE
Storage conditions
Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.
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