Beschrijving
Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.
Specifications
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Nature name |
Nature Details |
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Product Alias |
MHC, KRAS, K-Ras 2, KRAS2, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KRAS1, KRAS2, NS, NS3, RASK2, GTPase Kras, KI-RAS, RALD |
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Species |
Human |
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Expression sequence |
Gly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M) and VVVGAVGVGK peptide |
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Accession |
NP_002107.3 (HLA-A*03:01)&P61769 (B2M)&VVGAVGVGK |
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Label |
C-His-Avi |
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Expression system |
HEK293 |
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Molecular weight |
Approximately 50.2 kDa.Due to glycosylation, the protein migrates to55-60 kDa based on Tris-Bis PAGE result. |
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purity |
> 95% as determined by SDS-PAGE and HPLC. |
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Endotoxin |
< 1.0 EU per μg by the LAL method. |
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preparation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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Appearance |
Lyophilized Powder |
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Biological Activity |
【SPR Data】Human KRAS G12V (HLA-A*03:01) Complex, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-KRAS G12V (HLA-A*03:01) Antibody with an affinity constant of 0.205μM as determined in SPR assay (Biacore T200). |
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Reconstitution method |
Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.。 |
Product Features
High purity, high activity, low endotoxin, high batch consistency
Application Cases
Product Data
Tris-Bis PAGE
Storage conditions
Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.
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