Beschrijving
This kit contains recombinant mouse IL-2, IL-4, IL-21, and BAFF for the in vitro polarization of mouse B cells.
IL-2 is typically added as an enhancing component to existing polarization systems. Its most important applications include synergizing with IL-4 to enhance IgG1/IgE class switching, and with TGF-β to enhance IgA class switching. In the early stages of the B cell response, it promotes clonal expansion and prepares cells for class switching; in later stages, it may influence the fate decision between plasma cells and memory B cells.
IL-4 is the core instructive factor for IgG1 and IgE class switching. In T cell-dependent responses, IL-4 is the most critical cytokine for inducing B cells to switch from expressing IgM/IgD to expressing IgG1 and IgE. Meanwhile, it is a potent B cell co-stimulatory proliferation factor, acting synergistically with BCR or CD40 signaling.
IL-21 induces B cell differentiation into plasma cells and antibody secretion.
BAFF promotes B cell survival and maturation.
Product Information
|
Product Information. |
Catalog Number |
Size |
|
Mouse B lymphocyte Polarization Cytokine Set |
92655ES10 |
10 μg |
|
92655ES50 |
50 μg |
|
|
92655ES60 |
100 μg |
Components
|
Component Cat No. |
Component Name |
92655ES10 |
92655ES50 |
92655ES60 |
Quantity |
|
92655ES-A |
Mouse IL-2 Protein |
10 μg |
50 μg |
100 μg |
1 |
|
92655ES-B |
Mouse IL-4 Protein |
10 μg |
50 μg |
100 μg |
1 |
|
92655ES-C |
Mouse IL-21 Protein |
10 μg |
50 μg |
100 μg |
1 |
|
92655ES-D |
Mouse BAFF Protein |
10 μg |
50 μg |
100 μg |
1 |
Product Includes
|
Component Cat No. |
Product Name |
Source |
Appearance |
|
90239ES |
Mouse IL-2 Protein |
HEK293 |
Liquid |
|
90241ES |
Mouse IL-4 Protein |
HEK293 |
Liquid |
|
90233ES |
Mouse IL-21 Protein |
HEK293 |
Liquid |
|
90641ES |
Mouse BAFF Protein |
HEK293 |
Lyophilized powder |
Reconstitution Method
|
Component Name |
Reconstitution Method |
|
Recombinant Mouse BAFF Protein |
Before opening, centrifuge to bring the contents to the bottom. Reconstitute with ultrapure water to a concentration of 0.1-1.0 mg/mL. Further dilution can be performed based on subsequent experiments. |
Performance Parameters
|
Species |
Mouse |
|
Tag |
No Tag |
|
Expression System |
HEK293 |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
Endotoxin |
< 0.01 EU per µg by the LAL method. |
|
Formulation |
PBS buffer. |
|
Appearance |
Liquid |
Storage
Store at -85 ~ -65°C. Valid for 1 year from the date of receipt.
After reconstitution, store at -85 ~ -65°C under sterile conditions for 3 months.
Product Features
High activity, high purity, high stability, low endotoxin.
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Het product is alleen bedoeld voor onderzoeksdoeleinden en is niet bedoeld voor therapeutisch of diagnostisch gebruik bij mensen of dieren. Producten en inhoud worden beschermd door patenten, handelsmerken en auteursrechten die eigendom zijn van
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