Beschrijving
This kit contains recombinant mouse IL-6, mouse TGF-β1, mouse IL-23, and mouse IL-1β, and is used to induce the differentiation of mouse naïve CD4+ T cells into Th17 cells. IL-6 and TGF-β1 act synergistically to activate the STAT3 signaling pathway. This is essential for overcoming TGF-β-induced Treg differentiation and shifting towards the Th17 differentiation program. They also induce the production of the autocrine factor IL-21, which further amplifies the Th17 response.
IL-23 is added during the middle to late stages of Th17 differentiation. By activating STAT3, it maintains the expression of RORγt and the IL-23 receptor, promotes the terminal differentiation and expansion of Th17 cells, and enhances their pathogenicity. In the absence of IL-23, the resulting Th17 cells may be unstable and prone to convert into other subtypes.
IL-1β is a potent enhancer. For mouse Th17 cells, adding IL-1β can significantly improve polarization efficiency (the proportion of IL-17A+ cells), especially when using serum-free media or when polarization efficiency is suboptimal.
These cytokines constitute the "instructive" microenvironment for Th17 differentiation and are key to the success of the experiment.
Product Information
|
Product Information. |
Catalog Number |
Size |
|
Mouse Th17 Cell Polarization Cytokine Set |
92654ES10 |
10 μg |
|
92654ES50 |
50 μg |
|
|
92654ES60 |
100 μg |
Components
|
Component Cat No. |
Component Name |
92654ES10 |
92654ES50 |
92654ES60 |
Quantity |
|
92654ES-A |
Mouse IL-6 Protein |
10 μg |
50 μg |
100 μg |
1 |
|
92654ES-B |
Mouse TGF-β1 Protein |
10 μg |
50 μg |
100 μg |
1 |
|
92654ES-C |
Mouse IL-23 Protein |
10 μg |
50 μg |
100 μg |
1 |
|
92654ES-D |
Mouse IL-1β Protein |
10 μg |
50 μg |
100 μg |
1 |
Product Includes
|
Component Cat No. |
Product Name |
Source |
Appearance |
|
90146ES |
Recombinant Mouse IL-6 Protein |
E.coli |
Lyophilized powder |
|
91701ES |
Recombinant Mouse TGF-β1 Protein |
HEK293 |
Liquid |
|
90226ES |
Recombinant Mouse IL-23 Protein |
HEK293 |
Lyophilized powder |
|
90140ES |
Recombinant Mouse IL-1β Protein |
E.coli |
Lyophilized powder |
Reconstitution Method
|
Component Name |
Reconstitution Method |
|
Recombinant Mouse IL-6 Protein |
Before opening, centrifuge first to bring the contents to the bottom. Reconstitute with sterile deionized water to a concentration of 0.1-1.0 mg/mL. It can be further diluted according to subsequent experiments. For long-term storage, it is recommended to add carrier protein (0.1% BSA) to the dilution buffer; aliquot into single-use amounts, store frozen at -80°C, and avoid repeated freeze-thaw cycles. |
|
Recombinant Mouse IL-23 Protein |
|
|
Recombinant Mouse IL-1β Protein |
Performance Parameters
|
Species |
Mouse |
|
Tag |
No Tag |
|
Expression System |
HEK293 |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
Endotoxin |
< 0.01 EU per µg by the LAL method. |
|
Appearance |
Lyophilized Powder |
Storage
Store at -85 ~ -65°C. Valid for 1 year from the date of receipt.
After reconstitution, store at -85 ~ -65°C under sterile conditions for 3 months.
Product Features
High activity, high purity, high stability, low endotoxin
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