Beschrijving
The human hematopoietic stem cell (HSC) expansion cytokine set contains recombinant human Flt3-Ligand, recombinant human SCF, recombinant human TPO, and recombinant human IL-3. These four cytokines are essential key growth factors for the in vitro culture of hematopoietic stem cells (HSCs). These factors promote the proliferation, survival, and differentiation of HSCs.
Flt3-Ligand is a growth factor that regulates the proliferation of early hematopoietic cells. It primarily acts on short-term hematopoietic stem cells and multipotent progenitor cells, prompting them to rapidly enter the cell cycle for expansion. By itself, it does not stimulate the proliferation of early hematopoietic cells, but rather acts synergistically with other cytokines to induce growth and differentiation.
SCF (Stem Cell Factor) is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. It can effectively inhibit the programmed cell death of HSCs in vitro. SCF itself has limited proliferative capacity, but it can "prime" the cells, bringing them into an activated state and greatly enhancing the effects of other factors (such as Flt3-L, TPO, and IL series). It is the cornerstone of almost all efficient expansion protocols. Furthermore, similar to its interaction with membrane-bound SCF in the bone marrow microenvironment, it helps HSCs maintain their undifferentiated state and homing ability.
TPO (Thrombopoietin) has a core function in maintaining the quiescence and self-renewal of long-term hematopoietic stem cells. In expansion cultures, the presence of TPO helps maintain cells in a more primitive state, reducing premature differentiation into lineage-committed progenitor cells, thereby preserving their long-term repopulation ability after transplantation. Although its primary function is to support megakaryocyte/platelet differentiation, its role at the early HSC stage is to maintain stemness.
IL-3 (Interleukin-3) is a potent broad-spectrum hematopoietic growth stimulator. It can stimulate the proliferation and differentiation of multipotent progenitor cells as well as lineage-committed progenitor cells of granulocytes, macrophages, eosinophils, and mast cells. Adding IL-3 to an expansion protocol can significantly increase the total cell yield because it drives the proliferation of a large number of differentiated progenitor cells.
Product Information
|
Product Information. |
Catalog Number |
Size |
|
Human Hematopoietic Stem Cell Expansion Cytokine Set |
92645ES10 |
10 μg |
|
92645ES60 |
100 μg |
Components
|
Component Cat No. |
Component Name |
92645ES10 |
92645ES60 |
Quantity |
|
92645ES-A |
Human FLT3-Ligand Protein |
10 μg |
100 μg |
1 |
|
92645ES-B |
Human SCF Protein |
10 μg |
100 μg |
1 |
|
92645ES-C |
Human TPO Protein |
10 μg |
100 μg |
1 |
|
92645ES-D |
Human IL-3 Protein |
10 μg |
50 μg |
1 |
Product Includes
|
Component Cat No. |
Product Name |
Source |
Appearance |
|
92279ES |
Human FLT3-Ligand Protein |
HEK293 Cells |
Lyophilized powder |
|
92251ES |
Human SCF Protein |
E.coli |
Lyophilized powder |
|
92261ES |
Human TPO Protein |
CHO Cells |
Liquid |
|
90104ES |
Human IL-3 Protein |
E.coli |
Lyophilized powder |
Reconstitution Method
|
Component Name |
Reconstitution Method |
|
Human FLT3-Ligand Protein |
Before opening, centrifuge first to bring the contents to the bottom. Reconstitute with sterile PBS to a concentration of 0.1-1.0 mg/mL; further dilution can be performed according to subsequent experiments. For long-term storage, it is recommended to add carrier protein (0.1% BSA) to the dilution buffer; aliquot into single-use amounts, store frozen at -80°C, and avoid repeated freeze-thaw cycles. |
|
Human SCF Protein |
Before opening, centrifuge first to bring the contents to the bottom. Reconstitute with sterile water to a concentration of 0.1-1.0 mg/mL; further dilution can be performed according to subsequent experiments. For long-term storage, it is recommended to add carrier protein (0.1% BSA) to the dilution buffer; aliquot into single-use amounts, store frozen at -80°C, and avoid repeated freeze-thaw cycles. |
|
Human IL-3 Protein |
|
|
Human TPO Protein |
Reconstitute the lyophilized protein with sterile deionized water. Concentration: Reconstitute to a concentration of 0.1-1.0 mg/mL. The solution can be further diluted for subsequent experiments. |
Product Properties
|
Species |
Human |
|
Tag |
No Tag |
|
Expression System |
HEK293 |
|
Purity |
> 95% as determined by SDS-PAGE |
|
Endotoxin |
< 1.0 EU per µg by the LAL method. |
|
Formulation |
0.2 µm filtered concentrated solution in PBS, pH 7.4. |
|
Appearance |
Lyophilized Powder |
Product Features
High activity, high purity, low endotoxin, high stability.
Storage
1. Store at -85 ~ -65°C, valid for 1 year from the date of receipt.
2. After reconstitution, store at 2~8°C under sterile conditions, valid for 7 days.
3. After reconstitution, store at -85 ~ -65°C under sterile conditions, valid for 3 months.
Documents:
Manuals:
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