Beschrijving
Tumor Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is a member of the colony-stimulating factor family and plays a critical role in immune regulation, hematopoiesis, and inflammation. GM-CSF is primarily produced by various cell types, including macrophages, T cells, B cells, and fibroblasts. It stimulates the proliferation, differentiation, and functional activation of granulocytes and monocytes within the hematopoietic lineage. By promoting the differentiation of multipotent stem cells in the bone marrow into granulocytes and monocytes, GM-CSF increases leukocyte counts and enhances their functionality, thereby strengthening the immune response and anti-infection capacity. Furthermore, GM-CSF plays a vital regulatory role in the activation and antimicrobial activity of macrophages and dendritic cells.
Features
High Purity: >95% purity to avoid immune responses caused by residual Host Cell Proteins (HCP) or DNA.
Ultra-Low Endotoxin: <10 EU/mg to minimize the risk of fever or inflammation.
Batch-to-Batch Consistency: Ensures stability and reproducibility in cell culture applications.
Regulatory Compliance: Meets regulatory requirements for cell therapy products and supports Investigational New Drug (IND) applications.
Animal-Origin Free (AOF): Free from animal viruses, pathogens, and exogenous factor contamination, ensuring maximum safety.
Components
|
Components No. |
Name |
91120ES10 |
91120ES50 |
91120ES80 |
|
91120 |
Recombinant Human GM-CSF GMP Protein |
10 μg |
50 μg |
1 mg |
Performance parameters
|
Synonyms |
Granulocyte/Macrophage Colony-Stimulating Factor, GM-CSF, CSF-2, MGI-1GM, |
|
Source |
E.coli. |
|
Sequence |
Ala18-Glu144 |
|
Molecular Weight |
Approximately 14 KDa |
|
Accession |
P04141 |
|
Tag |
No Tag |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
＜0.1 EU/μg protein as determined by LAL method. |
|
Sterility |
Negative. |
|
Biological Activity |
Human GM-CSF GMP Protein determined by a cell proliferation assay using human TF-1 cells . The specific activity of＞5× 10^6 IU/mg. |
|
Mycoplasma |
Negative. |
|
Vitro Virus |
Negative. |
|
Host Cell Protein |
Less than 0.5 ng/ug when tested by ELISA. |
|
Host Cell DNA |
Less than 20 ng/mg when tested by qPCR. |
|
Formulation |
0.22 μm filtered concentrated solution in PBS. |
|
Physical Appearance |
Lyophilized Powder |
Storage
This product should be stored at -25~-15℃ for 3 years. After reconstitution, store at -85 to -65°C. Valid for 3 months.
Figure
1. High purity
SDS- PAGE
Figure 1. Human GM-CSF Protein on SDS-PAGE under reduced condition. The purity is greater than 95%.
SEC-HPLC
Figure 2. The purity of Human GM-CSF is greater than 95% as determined by SEC-HPLC.
Cell Based Assay
Figure 3. Human GM-CSF GMP Protein determined by a cell proliferation assay using human TF-1 cells . The specific activity of＞5× 10^6 IU/mg.
Documents:
Safety Data Sheet
Manuals
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