Beschrijving
Interleukin-34 (IL-34) is a cytokine with a molecular weight of 23.98 kDa, consisting of 216 amino acid residues. IL-34 is a ligand for the colony-stimulating factor 1 receptor (CSF-1R) and is primarily secreted by the adrenal glands, frontal lobe, and cortex. It plays a crucial role in the proliferation, survival, differentiation, and migration of myeloid cells. These functions are mediated by IL-34 activating the ERK1/2, JAK, and PI3K/AKT signaling pathways.
Specifications
|
Cat.No. |
90297ES08 / 90297ES20 / 90297ES60 / 90297ES76 |
|
Size |
5 μg / 20 μg / 100 μg / 500 μg |
Components
|
Name |
90297ES08 |
90297ES20 |
90297ES60 |
90297ES76 |
|
HiActi™ Recombinant Mouse IL-34 Protein ,His Tag |
5 μg |
20 μg |
100 μg |
500 μg |
Performance parameters
|
Synonyms |
interleukin 34, C16orf77, IL34 |
|
Species |
Mouse |
|
Expression Sequence |
MNEPLEMWPLTQNEECTVTGFLRDKLQYRSRLQYMKHYFPINYKISVPYEGVFRIANVTRLQRAQVSERELRYLWVLVSLSATESVQDVLLEGHPSWKYLQEVETLLLNVQQGLTDVEVSPKVESVLSLLNAPGPNLKLVRPKALLDNCFRVMELLYCSCCKQSSVLNWQDCEVPSPQSCSPEPSLQYAATQLYPPPPWSPSSPPHSTGSVRPVRAQGEGLLP with polyhistidine tag at the C-terminus. |
|
Accession |
Q6ZMJ4 |
|
Tag |
C-His |
|
Expression System |
E.coli |
|
Molecular Weight |
The protein has a calculated MW of 26.17 kDa. The protein migrates as 30 kDa under reducing condition |
|
Purity |
>98% as determined by SDS-PAGE. |
|
Endotoxin |
<0.1 EU per 1 μg of the protein by the LAL method. |
|
Formulation |
The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. |
|
Appearance |
Lyophilized Powder |
|
Biological activity |
Measure by its ability to induce human peripheral blood monocytes (PBMCs) proliferation. The ED₅₀ for this effect is <30 pg/mL. |
|
Reconstitution Instructions |
Briefly centrifuge the vial at 3000 rpm for 5 minutes before opening. It is recommended to reconstitute the lyophilized powder with sterile water to a concentration of no less than 100 μg/mL, and let it stand for at least 20 minutes to ensure complete dissolution. The reconstituted solution can be further diluted and aliquoted. When performing further dilution and aliquoting, a carrier protein (0.1% BSA, 10% FBS, or 5% HSA) must be added. For serum-free experimental requirements, a 5% trehalose solution can be used as an alternative carrier. |
Storage
Store at -25 to -15°C. Valid for 1 year from receipt. After reconstitution, store at 2 to 8°C for up to 7 days, or at -85 to -65°C for up to 3 months. To avoid repeated freeze-thaw cycles, we recommend aliquoting the protein upon first use.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves, for your safety.
Documents
Safety Data Sheet
Manuals:
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FAQ
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