Beschrijving
The Hieff™ Endo-free Plasmid Midi Kit enables rapid purification of 100–500 µg of high-purity, high-copy plasmid DNA from 30–70 mL of E. coli LB (Luria-Bertani) culture. The spin column utilizes a proprietary silica-based matrix developed by our company, combined with a unique lysis buffer formulation, to maximize recovery of high-quality plasmid DNA. A specialized endotoxin-removal process ensures extremely low endotoxin levels (<0.1 EU/µg DNA), resulting in excellent performance in cell transfection. The purified plasmid is also directly suitable for a wide range of downstream molecular biology applications, including restriction digestion, transformation, PCR, in vitro transcription, sequencing, and more.
Features
High Stability: Specially designed silica membranes in spin columns ensure minimal variation between columns and excellent reproducibility.
High Yield: Quickly and easily extract 100–500 µg of high-copy plasmid DNA from 30–70 mL E. coli LB culture.
Low Endotoxin: Unique formulation reduces endotoxin to <0.1 EU/µg DNA, ideal for efficient cell transfection.
Components
|
Components No. |
Name |
19023ES02 |
19023ES20 |
|
|
Part I |
19023-A |
RNase A (10 mg/mL) |
50 µL |
500 µL |
|
Part II |
19023-B |
Endotoxin Removal Solution ER (ER Solution E2) |
1.8 mL |
18 mL |
|
19023-C |
RS Buffer* (RS Buffer E2*) |
5 mL |
50 mL |
|
|
Part III |
19023-D |
AC Buffer (AC Buffer E2) |
1 mL |
10 mL |
|
19023-E |
Hieff™ DNA Column E2 |
2 columns |
20 columns |
|
|
19023-F |
15 mL Collection Tube E2 |
2 tubes |
20 tubes |
|
|
19023-G |
Lysis Buffer LB (LB Buffer E2) |
5 mL |
50 mL |
|
|
19023-H |
Binding Buffer BD (BD Buffer E2) |
5 mL |
50 mL |
|
|
19023-I |
Protein Removal Buffer PL* (PL Buffer E2*) |
4 mL |
40 mL |
|
|
19023-J |
Wash Buffer W* |
2.5 mL |
25 mL |
|
|
19023-K |
Elution Buffer |
2 mL |
20 mL |
|
Storage
Part I: Store at –25°C to –15°C
Part II: Store at 2–8°C
Part III: Store at room temperature
Valid life: 2 years
Documents:
Safety Data Sheet
Manuals
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FAQ
Het product is alleen bedoeld voor onderzoeksdoeleinden en is niet bedoeld voor therapeutisch of diagnostisch gebruik bij mensen of dieren. Producten en inhoud worden beschermd door patenten, handelsmerken en auteursrechten die eigendom zijn van
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