This product is a multiplex qPCR premix utilizing the company's next-generation antibody-based hot-start Taq enzyme.

Powered by Yeasen's ZymeEditor™ enzyme engineering platform, researchers performed comprehensive directed evolution of Taq DNA polymerase through structural analysis, multi-source sequence alignment, site-directed mutagenesis, random mutagenesis screening, stress testing, and enzyme fragment recombination. Using MTPS high-throughput screening technology, candidates were evaluated across thermal stability, affinity, polymerase activity, and exonuclease activity. The resulting Taq DNA polymerase exhibits enhanced inhibitor tolerance and fast processive synthesis. The molecular diagnostics raw material R&D platform further optimized the qPCR reaction system through extensive clinical sample testing and comparison with market alternatives, officially launching this qPCR premix specifically designed for African swine fever virus (ASFV) detection. Customized kit collaborations with ASFV primers and probes are also available.

Feature

• Compatibility: Optimized for ASFV nucleic acid detection, particularly compatible with Hongshi and Tianlong real-time PCR instruments.
• Sensitivity: Demonstrated higher detection rate and sensitivity than comparable market products across hundreds of clinical samples.
• Fast: Results available within 20 minutes.
• Stability: Premixed primers and probes remain stable after 7 days at 37°C and 20 freeze-thaw cycles.
• dUTP/UDG contamination prevention system: Incorporates a dUTP/UDG system that effectively prevents aerosol contamination.

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Case Study 1: ASFV Clinical Sample Detection

Figure 1. Amplification curves of 45 ASFV clinical samples.

Table 1. Ct value statistics of 45 ASFV clinical samples.

Results: Forty-five clinical samples were tested in parallel using commercially available qPCR premixes for African swine fever virus (ASFV) detection. The results showed that Yeasen 16967 achieved a higher ASFV detection rate, stronger fluorescence signals, and better detection performance for low-concentration samples.

Case Study 2: Sensitivity Detection

Table 2. Sensitivity comparison of ASFV detection using qPCR premixes from different manufacturers.

Results: Two serially diluted African swine fever virus (ASFV) nucleic acid samples at the limit of detection (LoD) were tested in parallel using comparable commercially available qPCR premixes for ASFV detection. The results showed that Yeasen 16967 demonstrated slightly better sensitivity and Ct values than comparable products on the market.

Case Study 3: Stability Test

Figure 2. Stability test of 16967 with premixed ASFV primers and probes after 7 days at 37°C.

Results: The 16967 premix containing African swine fever virus (ASFV) primers and probe was incubated at 37°C for 7 days and compared with the reagent stored routinely at −20°C. Both reagents were used to amplify the ASFV positive control, nucleic acid at the sensitivity-level concentration, and NTC. The results showed no significant differences in amplification curve profiles, Ct values, or detection rates between the two storage conditions. In addition, no nonspecific amplification was observed after thermal acceleration, indicating that the fully premixed product has good stability.

Storage

Store at -25°C to -15°C, valid for 18 months.

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