Beschrijving
HiSalt T4 DNA Ligase is a salt-tolerant mutant of T4 DNA ligase that catalyzes the formation of phosphodiester bonds between adjacent 5′ phosphate and 3′ hydroxyl termini on double-stranded DNA or RNA. Compared with the wild-type T4 DNA ligase, this enzyme exhibits stronger tolerance under high-salt conditions. Its catalytic activity depends on ATP as a cofactor and remains effective at salt concentrations up to 300 mM. It can ligate both blunt and cohesive ends, repair double-stranded DNA, and seal single-strand nicks in some DNA/RNA hybrid strands.
|
Cat.No. |
Size |
Price |
|
14967ES20 |
20 KU |
$65 |
|
14967ES60 |
100 KU |
$265 |
Components
|
Components No. |
Name |
14967ES20 |
14967ES60 |
|
14967-A |
HiSalt T4 DNA Ligase (400 U/μL) |
50 μL |
250 μL |
|
14967-B |
10×T4DL Reaction Buffer |
1 mL |
3×1 mL |
Specifications
|
Source |
Recombinant expression in E. coli, derived from engineered HiSalt T4 DNA Ligase |
|
Concentration |
400 U/μL |
|
Purity |
≥95% |
|
Tag |
None |
|
Enzyme Storage Buffer |
10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol (pH 7.4 @ 25°C) |
Shipping and Storage
Ship with ice pack. Store at -25~-15°C. Valid for 2 year.
Application
1. Ligation of restriction endonuclease-digested DNA fragments.
2. Ligation of linkers and adapters to the blunt ends of DNA fragments.
Figure
Salt Tolerance on Cohesive-End Substrates
Figure 1. Salt tolerance of 14967ES T4 DNA Ligase.
Relative activity was measured at increasing NaCl concentrations. 14967ES (red triangles) maintained high ligation activity up to 750 mM NaCl, while the wild-type T4 DNA ligase (black triangles) showed a marked activity decline above 100 mM NaCl.
Documents:
Safety Data Sheet
Manuals
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