Beschrijving
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the 5'-phosphate and 3'-hydroxyl termini of adjacent nucleotides in double-stranded DNA or RNA with either cohesive or blunt ends. This reaction requires ATP as a cofactor. Additionally, T4 DNA Ligase can repair single-strand nicks in double-stranded DNA, double-stranded RNA, or DNA/RNA hybrids.
Specifications
|
Cat.No. |
14966ES40 / 14966ES42 |
|
Size |
40 KU / 400 KU |
|
Concentration |
400 U/μL |
|
Source |
Recombinant E. coli strain carrying the T4 DNA ligase gene. |
|
Enzyme Activity Definition |
One unit is defined as the amount of DNA ligase required to achieve 50% ligation of 100 ng of cohesive-end DNA fragments in 50 µL of 1X DNA Ligase Reaction Buffer with incubation for 30 minutes at 23°C. |
Components
|
Components No. |
Name |
14966ES40 |
14966ES42 |
|
14966-A |
Premium T4 DNA Ligase (400 U/μL) |
100 µL |
1 mL |
|
14966-B |
2 × Rapid Ligation Buffer |
1 mL |
10 mL |
|
14966-C |
10 × T4 DNA Ligase Buffer |
1 mL |
10 mL |
[Note]:
① High-concentration Premium T4 DNA Ligase combined with 2× Rapid Ligation Buffer significantly enhances the speed and efficiency of blunt-end ligation. Therefore, prolonged incubation (>10 minutes) is not recommended, as it may substantially reduce the transformation efficiency of the ligation products.
② The 10× T4 DNA Ligase Buffer does not contain PEG and is compatible with standard ligation protocols that do not specify the use of rapid ligation buffer.
Storage
This product should be stored at -25~-15℃ for 2 years.
Notes
1. For your safety and health, wear a lab coat and disposable gloves while handling this product.
2. This product is intended for research use only.
3. It is normal for a small amount of precipitate to appear when the buffer is thawed. Simply invert and mix well before use.
Documents:
Safety Data Sheet
Manuals
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