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LwaCas13a Nuclease is an RNA-guided RNA endonuclease derived from Leptotrichia wadei. Its activity depends on an RNA-mediated recognition mechanism. In the presence of a suitable protospacer flanking site (PFS) sequence on the target single-stranded RNA (ssRNA), LwaCas13a specifically recognizes and cleaves the target ssRNA under the guidance of a CRISPR RNA (crRNA).In addition, LwaCas13a possesses target-dependent trans-cleavage (collateral cleavage) activity. This activity is triggered only after the formation of a ternary complex consisting of LwaCas13a, crRNA, and the target ssRNA. Once activated, the enzyme not only efficiently cleaves the target RNA but also non-specifically degrades any single-stranded RNA present in the reaction system. Leveraging this signal-amplification property, LwaCas13a is widely used in the development of rapid nucleic acid detection kits and holds significant value in the field of molecular diagnostics.
Specifications
|
Source |
Recombinant expression in Escherichia coli of the Cas13a gene from Leptotrichia wadei |
|
Molecular weight |
138.5 kDa |
|
Concentration |
10 μM |
|
Purity |
≥95% |
|
Tag |
None |
|
Storage buffer |
25 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol (pH 8 at 25℃) |
Components
|
Component No. |
Component |
14709ES77 |
14709ES88 |
|
14709-A |
LwaCas13a Nuclease (10 μM) |
70 μL |
350 μL |
|
14709-B |
10× Cas13a Reaction Buffer |
1 mL |
5 × 1 mL |
Storage
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