Creatine kinase (CK) is mainly found in the cytoplasm and mitochondria. It is an important kinase directly related to intracellular energy metabolism, muscle contraction, and ATP regeneration. CK mainly catalyzes the conversion of adenosine diphosphate (ADP) and phosphocreatine to ATP and creatine, playing a critical role in rapid ATP regeneration in cells with high energy demand. In RPA technology, ATP generated by creatine kinase-catalyzed hydrolysis supplies energy, thereby altering the conformation of the recombinase (T4 UvsX)-primer complex, dissociating the recombinase to expose the 3′ end of the primer, and thus promoting the binding of strand-displacement Bsu DNA polymerase to the primer to initiate DNA amplification.

Product Information

Component Information

Feature

• Creatine kinase has been used as a component of translation buffer in in vitro translation assays and as a component of energy-regeneration mixtures.
• Storage buffer: 50 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, 2 mM DTT, 50% glycerol, pH 7.5 at 25°C.
• The molecular weight of this protein is 44 kDa.

Storage

Store at -25°C to -15°C. The shelf life is 1 year.

Published Literature

(1) Engineering UvsY solubility through local hydrophobic cluster disruption enables enhanced recombination mediator activity

Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | DOI: 10.1016/j.ijbiomac.2026.151408 | IF: 8.7

Documents:

Safety Data Sheet

14502_MSDS_HB260819

Manuals:

14502_Manual_HB20260818