Beschrijving
9°N DNA Polymerase is derived from the 9°N-7 gene of the Thermococcus genus. Compared to other commercially available polymerases, its core advantages lie in its high fidelity, robust stability, efficient amplification, and broad applicability. It is particularly effective in precisely addressing error issues in DNA storage, thereby reducing costs and enhancing data reliability. As a naturally high-fidelity enzyme, it is specifically adapted for applications in digital information storage using DNA.
Specifications
|
Cat.No. |
14470ES60 / 14470ES76 |
|
Size |
100 U/ 500 U |
|
Concentration |
2 U/μL |
|
Source |
Recombinant E. coli strain containing the 9°N-7 gene from Thermococcus. |
Components
|
Components No. |
Name |
14470ES60 |
14470ES76 |
|
14470-A |
9°Nm DNA Polymerase (2 U/μL) |
50 μL |
250 μL |
|
14470-B |
10×Taq Reaction Buffer |
500 μL |
1.5 mL |
Storage
This product should be stored at -25~-15℃ for 2 years.
Instructions
1. Prepare the following reaction system and mix thoroughly:
|
Component |
Volume(μL) |
|
10×Taq Reaction Buffer |
2 μL |
|
dNTPs (2.5 mM each) |
1.6 μL |
|
Template DNA |
10-100 ng |
|
Primers (10 μM) |
1 μL |
|
9°Nm DNA Polymerase (2 U/μL) |
0.5-1 μL |
|
ddH2O |
Adjust to 20 μL |
[Note]: The above reaction system can be scaled up or down proportionally according to actual conditions.
2. After mixing the above system, set the reaction program: (95°C denaturation for 30 sec, annealing at Tm–5°C for 30 sec, extension at 65–72°C for 1 min/kb) for 25–35 cycles;
3. Sample for identification or analysis.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves，for your safety.
3. Avoid repeated freeze-thaw cycles, and use nuclease-free pipette tips and centrifuge tubes during the experiment.
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