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Bsu DNA polymerase (Large Fragment) is derived from Bacillus subtilis. This enzyme retains the 5′-to-3′ polymerase activity of Bsu DNA polymerase I but lacks the 5′-to-3′ exonuclease domain. The large fragment itself lacks 3′-to-5′ exonuclease activity. It is a DNA isothermal amplification polymerase with strand-displacement activity and is mainly used in Recombinase Polymerase Amplification (RPA).
Product Information
|
Catalog No. |
14415ES72 / 14415ES80 / 14415ES90 / 14415ES97 |
|
Size |
2,500 U / 5,000 U / 50,000 U / 250,000 U |
|
Unit Definition |
One unit (U) is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble material within 30 min at 37°C. |
|
Storage Buffer |
25 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C. |
|
Heat Inactivation |
75°C for 20 min. |
Component Information
|
Product Name |
14415ES72 |
14415ES80 |
14415ES90 |
14415ES97 |
|
Bsu DNA Polymerase (Large Fragment, 50 U/μL) |
50 μL |
100 μL |
1 mL |
5 × 1 mL |
Feature
- Due to the lack of 3′-to-5′ exonuclease activity, Bsu DNA polymerase (Large Fragment) cannot remove unpaired 3′ overhangs and is therefore not suitable for generating blunt ends.
- At 25°C, Bsu DNA polymerase (Large Fragment) retains 50% activity, which is twice that of Klenow Fragment (3′-to-5′ exo-) at the same temperature.
- Storage buffer: 25 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C.
- Heat inactivation: 75°C for 20 min.
Storage
Store at -25°C to -15°C. The shelf life is 2 years.
Documents:
Safety Data Sheet
Manuals:
Storage
FAQ
Citations
Documents
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