Beschrijving
This kit integrates a visible-light LAMP indicator into the formulation. The reaction buffer contains dATP, dCTP, dGTP, and dUTP—together with additional components essential for Bst II DNA Polymerase amplification—as well as a pH-sensitive indicator. The kit also provides glycerol-free Bst II DNA Polymerase, UDGase, and reverse transcriptase (RT enzyme), and is supplied with a lyophilization protectant, enabling direct preparation of lyophilized reaction systems on a standard freeze-dryer. The reagent delivers high amplification efficiency and sensitivity, with amplification results readable by the unaided eye: positive reactions turn orange-yellow while negative reactions remain magenta, providing strong visual contrast.
Product Information
|
Product Name |
Cat. No. |
Size |
|
RT-LAMP pH Sensitive Dye Chromogenic Version Freeze-Dryable Reagent |
13920ES60 |
100 T |
|
13920ES80 |
1,000 T |
|
|
13920ES92 |
10,000 T |
Product Components
|
Component No. |
Component Name |
13920ES60 (100 T) |
13920ES80 (1,000 T) |
13920ES92 (10,000 T) |
|
13920-A |
2.5× pH Sensitive Reaction Buffer |
1 mL |
10 mL |
100 mL |
|
13920-B |
Glycerol-free RT Enzyme Mix |
50 μL |
500 μL |
5 mL |
|
13920-C |
Glycerol-free Bst Enzyme |
30 μL |
300 μL |
3 mL |
|
13920-D |
Lyophilization Protectant |
500 μL |
5 mL |
50 mL |
Applications
Features
• One-step isothermal RNA amplification: visual colorimetric readout eliminates the need for electrophoresis or fluorescence instrumentation.
• Rapid turnaround: pathogen status can be determined in as little as 30 minutes after sample loading.
• Flexible workflow: a constant-temperature water bath or standard PCR thermal cycler (60–65°C) is sufficient—no specialized LAMP instrument required.
• Lyophilization-ready: glycerol-free enzymes and a dedicated lyophilization protectant enable direct preparation of room-temperature-stable lyophilized kits.
• High contrast endpoint: orange-yellow (positive) versus magenta (negative) for unambiguous visual interpretation, suitable for high-throughput population screening.
Storage
Part I — Components 13920-A and 13920-D [2.5× pH-Sensitive Reaction Buffer / Lyophilization Protectant] are shipped on dry ice and should be stored at -25 to -15°C with a shelf life of 6 months.
Part II — Components 13920-B and 13920-C [Glycerol-free RT Enzyme Mix / Bst Enzyme] are shipped with ice packs and should be stored at 2 to 8°C with a shelf life of 3 months.
FAQ
Q: Can 13920ES be used directly without lyophilization?
Yes. Lyophilization is optional; the kit may be used directly as a liquid formulation without any loss of performance.
Q: The 13920ES protocol includes an 85°C inactivation step for 5 minutes. Should the tube be removed after this inactivation step, or after the 30-minute reaction at 60–65°C, for color observation?
A: The tube should be removed for color observation only after the 85°C inactivation step (5 minutes) has been completed.
Documents:
Safety Data Sheet
Manual
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