2×Hieff Unicon™ Multiplex ARMS qPCR Mix has strong blocking ability, enabling effective ARMS-PCR-mediated genotyping. The buffer and Taq DNA polymerase are specifically optimized and already contain the ions and dNTPs required for amplification — no additional components are needed.

Feature

High selectivity : capable of detecting a wide range of mutation types;

High sensitivity : with mutant-specific primers, mutations can be detected at a frequency as low as 0.05% in DNA at 10 ng/µL

Easy interpretation : results are direct and objective, with no manualΔCt calculation required

Specifications

Components

Figures

1. Superior Blockage Efficiency

The assay successfully distinguishes wild-type from mutant genes. Testing with 5 μL template input (10 ng/μL wild-type DNA and equivalent copy number of mutant plasmid) shows no amplification for wild-type templates, or a Ct value difference (ΔCt) of ≥ 6–8 cycles between wild-type and mutant targets.

2. High Sensitivity (0.05% LOD)

The kit achieves a detection limit of 0.05%. In validation tests using a 50% mixture standard (10 ng/μL wild-type DNA spiked with mutant plasmid KRAS G13D), the product demonstrated robust performance at the 0.05% mutant concentration.

3. Excellent Stability

1)Freeze-Thaw Stability: Reagent (Cat. #13755) maintains amplification and blockage efficiency after 10 freeze-thaw cycles.

2)Thermal Stability: No impact on performance (tested at locus L858R) after storage at 37°C for 7 days.