Beschrijving
This kit is a DNA enzyme digestion library preparation reagent designed for the third-generation Nanopore sequencing platform. This product uses high-quality fragmentation enzymes, gets rid of the cumbersome ultrasonic process, simplifies the operation process, and combines the fragmentation module with the end repair/addition A module, which greatly reduces the time and cost of library preparation. It can be applied to 50 ng-1 μg of conventional animal and plant genomes, microbial genomes and other samples, and realize DNA fragmentation, end repair and A-tail addition reactions in a single tube.
Features
- Easy to operate: One-tube reaction, DNA fragmentation, end repair and A-tail addition reactions can be performed in a single tube
- Wide compatibility: Applicable to conventional animal and plant genomes, microbial genomes, FFPE and other samples
- Easier operation process: The fragmentation module and the end repair/A-tail addition module are combined into one, reducing the time and cost of library construction
- Compatible with automated equipment: The reagents are in Mix form and are compatible with automated library construction equipment
Specifications
|
Cat.No. |
13309ES08 / 13309ES24 / 13309ES96 |
|
Size |
8 T / 24 T / 96 T |
Components
|
Component No. |
Name |
13309ES08 |
13309ES24 |
13309ES96 |
|
13309-A |
Smearase Buffer |
80 μL |
240 μL |
960 μL |
|
13309-B |
Smearase Enzyme |
40 μL |
120 μL |
480 μL |
Shipping and Storage
This product should be stored at -25~-15℃ for one year.
Figures
A. DNA fragment test
Figure 1. DNA fragment detection of different reaction time
Template: 293 gDNA, input DNA 500 ng. According to the recommended fragmentation time and magnetic bead purification ratio, 4-20 Kb fragments can be obtained.
B. higher long fragment read rate
Documents:
Safety Data Sheet
Manuals
13309_Manual_Ver.EN20250512.pdf
Citations & References:
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FAQ
Het product is alleen bedoeld voor onderzoeksdoeleinden en is niet bedoeld voor therapeutisch of diagnostisch gebruik bij mensen of dieren. Producten en inhoud worden beschermd door patenten, handelsmerken en auteursrechten die eigendom zijn van
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