Cat.No.

96267ES25 / 96267ES60 / 96267ES76

Size

25 μg / 100 μg / 500 μg

Name

96267ES25

96267ES60

96267ES76

Recombinant Cynomolgus IL-1R3/IL-1 RAcP Protein,His Tag,Ultra Low Endotoxin

25 μg

100 μg

500 μg

Synonyms

IL1RAP;IL-1RAP;C3orf13;IL-1 R3;IL-1 RAcP

Species

Cynomolgus

Expression Sequence

Ser21-Cys362

Accession

G7NYP7

Tag

C-His

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 40.49 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.

Purity

> 95% as determined by SDS-PAGE and SEC-HPLC

Endotoxin

Less than 0.01 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological activity

ELISA Data 1:Immobilized Cynomolgus IL-1R3, His Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 2.6 ng/ml determined by ELISA .

Reconstitution Method

Dissolve the lyophilized protein in distilled water. For vials containing 25 μg or 100 μg, we recommend reconstituting to a concentration of 0.5 mg/mL. For larger quantities (500 μg or 1 mg), a concentration of 1 mg/mL is suggested. During reconstitution, avoid vigorous vortexing or shaking. Aliquot the reconstituted solution as needed and store at -80°C. Avoid repeated freeze-thaw cycles.