Interleukin-3 (IL-3) is a hematopoietic growth factor that promotes the survival, differentiation, and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil, and mast cell lineages. IL-3 is produced by T cells, mast cells, and eosinophils, and can enhance thrombopoiesis, phagocytosis, and antibody-mediated cytotoxicity. Its ability to activate mononuclear cells suggests that IL-3 may have additional immunomodulatory roles. Many IL-3 activities depend on co-stimulation with other cytokines.

Recombinant IL-3 is a 15.0 kDa recombinant protein containing 133 amino acid residues. This product is expressed in E. coli and provided as a lyophilized powder. It features high activity, high purity, low endotoxin, and is tag-free.

Specifications

Components

Performance parameters

Storage

Store at -25~-15°C, valid for 3 years.After reconstitution, store at 2~8°C, valid for 30 days. After reconstitution, store at -85~-65°C, valid for 3 months.

Reconstitution Method

It is recommended to reconstitute Human IL-3 in sterile PBS to a concentration of 0.1-1.0 mg/mL. Avoid repeated freeze-thaw cycles as much as possible.

Product Data

SDS- PAGE

Human IL-3 on SDS-PAGE under reduced condition. The purity is greater than 95%.

Cell Based Assay

The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.7 × 10⁶ IU/mg.

Notes

1. This product is for research use only.

2. Please operate with lab coats and disposable gloves,for your safety.

Documents

Safety Data Sheet

90212_MSDS_HB260612

Manuals:

90212_Manual_HB20260611