Flt-3 Ligand (Fms-related tyrosine kinase 3 ligand, FLT3L) is a key cytokine supporting the proliferation, survival, and maintenance of early hematopoietic progenitor cells and dendritic cells. In HSC expansion systems, FLT3L promotes the self-renewal and output of multipotent progenitor cells. In iPSC-to-HSC differentiation protocols, it acts during the early hematopoietic specification stage, supporting the emergence of hemogenic endothelial cells and the subsequent formation of hematopoietic progenitor cells.

Product Properties

Features

• High purity: >95%, preventing immune reactions caused by residual host cell protein (HCP) or DNA.
• Ultra-low endotoxin: <10 EU/mg, minimizing the risk of fever or inflammation.
• Batch-to-batch consistency: ensuring stability and reproducibility in cell culture.
• Regulatory compliance: meeting regulatory requirements for cell therapy products and supporting IND submissions.
• Animal-origin-free: free of animal viruses, pathogenic agents, and adventitious agent contamination, ensuring high safety.

Applications

SDS-PAGE

Figure 1. The purity is greater than 95% as determined by SDS-PAGE under reduced condition.

Bioactivity

Figure 2. The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 2.0 ng/mL.

Storage

Store at -25 to -15°C, valid for 3 years. After reconstitution, store at -85 to -65°C, valid for 3 months.