This product is a single-tube, probe-based RT-qPCR master mix specifically formulated for one-step quantitative PCR assays using RNA templates, suitable for both singleplex and multiplex detection. Reverse transcription and qPCR are performed in the same reaction tube, simplifying workflow and minimizing the risk of contamination.

The kit incorporates a next-generation antibody-mediated hot-start Taq DNA polymerase, aptamer-modified reverse transcriptase, and a specialized buffer system optimized for one-step, fully pre-mixed RT-qPCR. It supports pre-mixing of primers and probes; users only need to add template RNA during setup, thereby reducing handling steps and reagent loss. Additionally, the formulation includes a dUTP/UDG system that remains active at room temperature, effectively mitigating the risk of aerosol-mediated carryover contamination.

The aptamer-modified reverse transcriptase ensures near-complete suppression of enzymatic activity at low or ambient temperatures while enhancing overall enzyme stability. Validation studies demonstrate that primer/probe pre-mixed reactions remain stable—showing no performance degradation—after storage at 25°C for 2 weeks or at 4°C for 4 weeks. Therefore, accelerated stability testing is recommended at temperatures such as 25°C, 16°C, and 4°C to best reflect real-world product performance.

Features

• Exceptional Premix Stability: Support prime&probe premixing, with proven stability at 37°C for up to 5 days.
• Supports fast protocols: Results available in as little as 30 minutes.
• Ultra-High Sensitivity and Specificity: Features a genetically engineered antibody-based hot-start Taq polymerase for enhanced sensitivity and specificity.
• dUTP/UDG Anti-Contamination System: Incorporates a dUTP/UDG system to effectively prevent aerosol contamination.
• Broad Instrument Compatibility: Compatible with a wide range of real-time PCR platforms, including Bio-Rad CFX96, ABI Q5, 7500, and SLAN.

Components

[Note]: “2× HifairTM SmartApt I Master Mix” is the abbreviated name for HifairTM SmartApt I One Step RT-qPCR Probe Master Mix, which contains dNTPs, dUTP, Mg²⁺, stabilizers, enhancers, reverse transcriptase, hot-start Taq DNA polymerase, and UDGase.

Storage

This product should be stored at -25~-15℃ for 1 year.

Application

Urgent testing (e.g., Infectious disease detection, Critical care diagnostics);

High-throughput testing(e.g., Disease screening, Pathogen screening, Virus detection);

Routine testin(e.g., Food microbiology detection ).

Figures

1. High Seneitivity & Superior fully premixed stability

Figure 1. Low-concentration detection performance of fully premixed 16914ES.

HPIV and RSV pseudovirus templates (1 copy/μL) were tested using a 25 μL respiratory assay. The 16914ES mix premixed with primers and probes was stored at 37°C for 5 days and compared with –20°C and freshly prepared controls. The amplification curves, fluorescence signals, Ct values, and detection rates showed no noticeable changes, confirming the strong stability and sensitivity of the fully premixed 16914ES.

Documents:

Safety Data Sheet

16914_MSDS_HB260130_EN.PDF

Manuals

16914_Manual_Ver.EN20260130.pdf