説明
T3 DNA Ligase is derived from bacteriophage T3 and is an ATP-dependent double-stranded DNA ligase. It efficiently catalyzes the formation of a phosphodiester bond between adjacent 5′-phosphate and 3′-hydroxyl groups in double-stranded DNA, with higher ligation efficiency for A/T-overhang termini compared to C/G-overhangs.
T3 DNA Ligase effectively ligates both cohesive (sticky) ends and blunt ends (blunt-end ligation efficiency can be enhanced by addition of PEG 6000). It also repairs single-strand nicks in double-stranded DNA or DNA–RNA hybrid duplexes. During nick sealing, the intact strand must be DNA, while the nicked strand may have any combination at its 5′ and 3′ termini—DNA–DNA, DNA–RNA, RNA–DNA, or RNA–RNA—all of which are substrates for T3 DNA Ligase. Consequently, this enzyme can be used to generate DNA–RNA or RNA–DNA chimeric linkages and to perform DNA template-directed RNA ligation for the synthesis of longer RNA fragments.
Additionally, T3 DNA Ligase exhibits higher tolerance to NaCl than T4 DNA Ligase.
Features
High Purity: Protein purity ≥99% (by SDS-PAGE)
Low Contaminants: Free of detectable exonuclease, nicking endonuclease, non-specific nuclease, and RNase activity
High Activity: Performance comparable to leading imported brands
Specifications
|
Cat NO. |
14965ES65 / 14965ES77 |
|
Size |
100 KU / 750 KU |
|
Concentration |
3000 U/μL |
|
Source |
Recombinant E. coli strain expressing gene 1.3 from Enterobacteria phage T3 |
|
Unit Definition |
One unit is defined as the amount of enzyme required to achieve 50% ligation of 100 ng of HindIII-digested λ DNA in a 20 μL reaction containing 1× T3 DNA Ligase Reaction Buffer, incubated at 25°C for 1 minute. |
Components
|
Components No. |
Name |
14965ES65 |
14965ES77 |
|
14965-A |
T3 DNA Ligase (3000 U/μL) |
34 μL |
250 μL |
|
14965-B |
2× T3 DL Buffer |
500 μL |
2 × 1.5 mL |
Storage
This product should be stored at -25~-15℃ for 2 years.
Application
Molecular cloning
Ligation of adapters or linkers to double-stranded DNA
Long-chain synthesis of antisense oligonucleotide (ASO) therapeutics
Circularization of linear DNA
Repair of nicked sites in double-stranded DNA
Formation of DNA–RNA chimeric junctions
DNA template–directed RNA ligation for the assembly of long RNA fragments
Figures
High Activity
Figure 1. Comparison of ligation efficiency between Yeasen T3 DNA Ligase and imported Supplier A.
Yeasen T3 DNA Ligase and Supplier A’s ligase were tested at different input amounts using 5 pmol sticky-end substrates. The results show that Yeasen T3 DNA Ligase achieves high-efficiency ligation comparable to the leading brand’s product.
Documents:
Safety Data Sheet
Manuals
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