Hieff NGS™ Human All Exon Probes are DNA probes designed based on the human reference genome GRCh38 and the latest patch sequences, providing true coverage of approximately 34.7 Mb of human CDS regions. They offer ultra-high coverage of protein-coding regions in the latest RefSeq, CCDS, and GENCODE databases, with full coverage of 7,144 exons under 30 bp. Recommended sequencing depth is 100× with 8 Gb of data.

Product Information

Component Information

Feature

DNA probes are stable and resistant to degradation.

Tiling design ensures comprehensive CDS coverage. Full coverage of 7,144 exons.

Capture efficiency > 80%.

Excellent coverage and uniformity performance.

Application Case

I. Using NCBI RefSeq CDS as reference to evaluate exon coverage from other manufacturers: YEASEN’s whole-exome panel provides complete coverage of small exons.

II. Hieff NGS™ Human All Exon Probes, with optimized probe design and hybridization system, deliver higher capture efficiency and superior uniformity compared to competitors. Flanking capture efficiency reaches 88%, 20× coverage at 99.8%, and 50× coverage at 94%.

Storage

Store at -85~-65°C. Valid for 1 year.

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