Descrizione
Survivin is a member of the Inhibitor of Apoptosis (IAP) gene family and contains a single BIR domain. Increased survivin expression at the G2/M checkpoint contributes to tissue homeostasis and differentiation during embryonic and fetal development. Increased expression in differentiated adult tissues, however, frequently results in unchecked cell survival, resulting in cancer. Survivin can protect cells from caspase-induced apoptosis activated in mitosis presumably by binding to Caspase-3, -7, and -9. Normally it is only expressed in actively proliferating cells, but is upregulated in most, if not all cancers; consequently, it has received significant attention as a potential oncotherapeutic target.
Specifications
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Nature name |
Nature Details |
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Product Alias |
Survivin, MHC, MHC I, BIRC5, API4, EPR-1, IAP4, survivin variant 3 alpha |
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Species |
Human |
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Expression sequence |
Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and LMLGEFLKL peptide |
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Accession |
A0A140T913 (HLA-A*02:01)&P61769 (B2M)&LMLGEFLKL |
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Label |
C-His-Avi |
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Expression system |
HEK293 |
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Molecular weight |
Approximately 50.5 kDa.Due to glycosylation, the protein migrates to53-60 kDa based on Tris-Bis PAGE result. |
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purity |
> 95% as determined by SDS-PAGE and HPLC. |
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Endotoxin |
< 1.0 EU per μg by the LAL method. |
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preparation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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Appearance |
Lyophilized Powder |
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Biological Activity |
【ELISA Data】Immobilized Human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 7.6ng/ml determined by ELISA |
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Reconstitution method |
Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.。 |
Product Features
High purity, high activity, low endotoxin, high batch consistency
Storage conditions
Store at -25 ~ -15℃, valid for 1 year after receipt.
After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.
Pagamento e sicurezza
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Indagine
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FAQ
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