Why Traditional qPCR&RT-qPCR Reagents Are Holding You Back
Conventional qPCR and RT-qPCR reagents often require multiple manual preparation steps—mixing primers, probes, enzymes, and buffers separately. This not only increases setup time, but also leaves room for human error. In fact, studies show that manual prep can result in error rates as high as 8–12%, depending on the operator’s experience and pipetting accuracy.
- Other limitations of traditional reagents include:
- Higher variability (CV ≥ 15%) leading to inconsistent results
- Lower sensitivity, with detection limits typically around 1–10 copies
- Longer workflows, often requiring 2–4 hours including pre-treatment
- Limited application to controlled lab settings with trained personnel
- High reagent waste and labor costs, with utilization rates below 80%
All-in-One Power: Fully Pre-Mixed, Fully Optimized
---Yeasen's Dual Guarantee: Premium Materials + Innovative Process
At Yeasen, every fully pre-mixed qPCR and RT-qPCR reagent is built on two pillars: top-quality raw materials and cutting-edge production technology. Our R&D team rigorously selects and tests each component to ensure purity, stability, and compatibility, while our advanced manufacturing process guarantees consistent, high-performance mixes—batch after batch.
This dual approach empowers researchers and diagnostic labs with ready-to-use solutions that simplify workflows, reduce errors, and deliver reliable, reproducible results. With Yeasen, you’re not just saving time—you’re gaining confidence in every run.
Figure 1. All-in-One vs. Traditional qPCR/RT-qPCR Reagents
1. Fully Pre-Mixed DNA Mix
(A) . Hifair™ Superpro Fast One Step RT-qPCR Master Mix (Cat#16913)
1)Product propertity
2)Ultra-Sensitive Amplification Performance
Figure 2. Reliable low-copy detection in multiplex qPCR using All-in-One premixed reagent
In a 4-plex system, Cat#16913ES reliably detected ultra-low concentration samples (250 copies/mL), demonstrating excellent sensitivity with a detection limit of at least 250 copies/mL.
3) Excellent Stability of All-in-One Premix
Figure 3. Stability evaluation of All-in-One premixed reagent
Yeasen’s premix (Cat#16913ES) was tested under 4°C, 37°C (7 days), and 50 freeze-thaw cycles. Compared to the -20°C control, ΔCt values stayed within ±0.5 and fluorescence deviations under 15% across four multiplex qPCR systems (3 quadruplex, 1 triplex), confirming its strong stability.
(B).Ultra-Clean, Low DNA residue--Hifair™ Superpro Fast One Step RT-qPCR Master Mix (Cat#16913ES)
1)Ultra-Clean Production Control
Figure 4. Ultra-Clean reagent production and quality control workflow
2) Ultra-Clean (Low Residual Contamination, Low host DNA residue)
Figure 5. Residual bacterial DNA and huaman DNA test
NTC (no template control) qPCR using Yeasen one-tube premix (Cat#16913ES) and conventional reagents was tested on E. coli, P. aeruginosa, MecA, and human DNA systems. No amplification was detected in 48-well NTCs with Yeasen premix, confirming ultra-low background bacterial and human DNA contamination.
3) Outstanding Fully Premix Stability
Figure 6.Thermal accelerated stability test(37℃ 14days)
4)Compatibility with rapid protocols
Figure 7. Rapid Protocol Performance Validation using the Hifair™ Superpro Fast One Step RT-qPCR Master Mix (Cat#16913)
Seven gradient samples (1–10⁶ copies/μL) were tested using both standard (66 min) and rapid (30 min) protocols on ABI Q5. The rapid protocol showed >99% efficiency, ΔCt within ±0.5, fluorescence deviation within ±10%, and typical S-shaped amplification curves.
2. Fully Pre-Mixed RT-qPCR Mix
Hifair™ Superpro Fast One Step RT-qPCR Master Mix (Cat#16913)
1）Excellent Amplification Performance
Validated against non-premixed systems, our full premix RT-qPCR delivers equally strong—or better—amplification performance.
Figure 8. Performance comparison of Yeasen’s fully premixed (Cat#16913) vs. conventional (Cat#16630) reagents across human respiratory, human genomic, and animal disease targets at 300 copies/mL.
Yeasen’s reagent matched the conventional reagent in performance and showed 1–2 cycle earlier Ct values than T brand, indicating better sensitivity at low target levels.
2）High Sensitivity
Figure 9. RT-qPCR targeting Influenza A and Influenza B was performed using the Hifair™ Superpro Fast One Step RT-qPCR Master Mix (Cat#16913).
The reagent successfully detected all low-concentration Influenza A and B templates (8/8), demonstrating excellent sensitivity.
3) Superior fully premixed stability: 37°C &4°C stability testing
Under 37°C accelerated and 4°C storage conditions, it maintains stable Ct values and fluorescence signals.
(A). Stability Evaluation of 37°C 5 Days
(B). Stability Evaluation of 4°C 14 Days
Figure 10. Stability assessment of Yeasen’s fully premixed reagent (Cat#16913ES).
The reagent was tested under accelerated conditions (37°C for 5 days) and refrigerated storage (4°C for 14 days), with performance compared to freshly prepared controls(-20℃）. Consistent amplification curves, Ct values, and detection rates were observed across human gene, pathogen detection targets, demonstrating high stability and sensitivity.
Product list
|
Category |
Name |
Cat. No. |
|
|
Probe qPCR Kit |
"one-tube" fully premixed reagents |
Hieff Unicon™ Pro Universal TaqMan Multiplex qPCR Mix (One-Tube, UDG Plus) |
16710ES |
|
"one-tube" fully premixed reagents, ultra-low DNA residue |
16713ES |
||
|
"one-tube" fully premixed reagents, ultra-low DNA residue |
Hieff Unicon™ Universal Superfast qPCR Mix |
16924ES |
|
|
Lyo-Ready qPCR reagent |
11893ES |
||
|
Probe RT-qPCR Kit |
Universal version reagent |
Hifair™ V2 Multiplex One Step RT-qPCR Probe Kit (UDG Plus, GC enhancer plus) |
16630ES |
|
"one-tube" fully premixed reagents |
11899ES |
||
|
"one-tube" fully premixed reagents |
16913ES |
||
|
"one-tube" fully premixed reagents |
16914ES |
||
|
Lyo-Ready RT-qPCR reagent |
Hifair™ Lyo-Ready I Multiplex One Step RT-qPCR Kit（UDG plus) |
16645ES |
|