Keterangan
|
Product Alias |
PRAME, OIP-4, OIP4, MAPE, |
|
Species |
Human |
|
Expression sequence |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYVDSLFFL peptide |
|
Accession |
P79603(HLA-A*02:01)&P61769(B2M)&ALYVDSLFFL |
|
Label |
C-His-Avi |
|
Expression system |
HEK293 |
|
Molecular weight |
The protein has a predicted MW of 50.60 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
Preparation |
Lyophilized from 0.22 μm filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
【ELISA Data】Immobilized Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA. |
|
Reconstitution method |
Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing. |
Product Features
High purity, high activity, low endotoxin, high batch consistency.
Storage
Store at -85 ~ -65℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.
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FAQ
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