Keterangan
Restriction endonuclease SmaI is derived from the recombinant expression of the SmaI gene in Serratia marcescens. It specifically cleaves double-stranded DNA to produce blunt ends. This product belongs to the fast-digest enzyme series and can rapidly complete the digestion of substrates such as plasmid DNA, PCR products, and genomic DNA within 15 minutes.
Applications
Molecular Cloning
Plasmid Linearization
Specifications
|
Recognition site |
5'-CCC↓GGG-3' 3'-GGG↓CCC-5' |
|
Concentration |
20 U/µL |
|
Inactivation Conditions |
One unit is defined as the amount of enzyme required to digest 1 µg of substrate DNA within 1 hour at 37°C in a total reaction volume of 50 µL |
|
Reaction conditions |
React at 37℃ for 15-30 min |
|
Inactivation conditions |
React at 65℃ for 20 min |
Components
|
Component No. |
Name |
15206ES84 |
15206ES92 |
|
15206-A |
SmaI (20 U/μL) |
100 μL |
500 μL |
|
15206-B |
10×SmaI Reaction Buffer |
1.25 mL |
1.25 mL |
Shipping and Storage
The products should be stored at -25℃ ~ -15℃ for 2 years.
Documents:
Safety Data Sheet
Manuals
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FAQ
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