Keterangan
BsaI is a Type IIs restriction endonuclease that recognizes non-palindromic sequences and cleaves outside of the recognition site. It is commonly used in Golden Gate assembly, digestion of plasmids to prepare linearized DNA fragments with poly(A/T/G/C) ends, and obtaining specific sticky ends.
Applications
Molecular Cloning
Plasmid Linearization
Specifications
|
Recognition site |
5'-GGTCTC(N)1↓-3' 3'-CCAGAG(N)5↑-5' |
|
Concentration |
20 U/µL |
|
Inactivation Conditions |
The amount of enzyme required to completely digest 1 µg of DNA within 1 hour at 37°C is defined as one unit of activity |
|
Reaction conditions |
React at 37℃ for 1 h |
|
Inactivation conditions |
React at 80℃ for 20 min |
Components
|
Component No. |
Name |
15204ES76 |
15204ES80 |
15204ES90 |
|
15204-A |
BsaI(20 U/μL) |
25 μL |
50 μL |
250 μL |
|
15204-B |
10× BsaI Buffer |
250 μL |
500 μL |
2.5 mL |
Shipping and Storage
The products should be stored at -25℃ ~ -15℃ for 2 years.
Documents:
Safety Data Sheet
Manuals
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FAQ
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