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From “suspension” to “adhesion”: complete operational guidelines for THP-1 Macrophage induction
Introduction of THP-1 cells
THP-1 cells are a peripheral blood cell line derived from a patient with human acute monocytic leukemia. Since its es...
One-Step Cell Identity Assurance: Species Authentication Made Simple
In the world of biopharmaceutical manufacturing, animal cell cultures serve as the essential “seeds” for life-saving biologics. Yet, one critical t...
New Arrival: ATP Vitality Detection Kit with More Stable Signal
In the vast field of biomedical research and application, cell viability detection has always been a focal point for researchers and clinical worke...
Th17 cell "deforming" guidelines: interesting tips for differentiation experiments that novices can also understand
I. Introduction of Th17 cells
Th17 cells are an important subset of CD4+ helper T cells, named for their predominant secretion of IL-17, regulate...
Choosing the Right Cell Proliferation Assay for Your Research
Cell proliferation is a central biological process that drives growth, development, and tissue repair. In research and drug discovery, measuring ce...
Troubleshooting Transfection: Common Problems and Solutions
I. Principles of Transfection
Q1: What is the difference between transfection and transduction?
Transfection: Introduction of naked or carrier-c...
Macrophage Depletion: Methods, Mechanisms, and Applications
1. Introduction to Macrophage Clearance
Macrophages are unique immune cells found in virtually every organ of the body, including avascular sites s...
Macrophage Depletion Procedures and Literature Case Analysis
1. Introduction to Macrophage Depletion
Macrophages are the only type of cell found in every organ of the body, including the epidermis, cornea, ...
Troubleshooting THP-1 Cell Adhesion During Culture
THP-1 cells are a human monocytic leukemia cell line cultured in suspension. They are widely used to model monocyte-tomacrophage differentiation. O...
What If There's No Positive Signal in ROS Detection? A Deep Dive Into ROS Probes
Reactive oxygen species (ROS) are oxygen-containing byproducts generated during cellular metabolism. Under physiological conditions, ROS levels are...
How Much Do You Know About TUNEL Staining? A Troubleshooting Guide for Abnormal Staining Results
During apoptosis, activated endonucleases cleave genomic DNA between nucleosomes. The commonly used TUNEL assay (TdT-mediated dUTP Nick End Labelin...
Protocol for Transfection of mRNA into Cells
1. Experimental Materials
HEK 293 cell: Cell confluency should reach 70%–90%.
mRNA: EGFP mRNA, 1000 bp (Concentration ≥ 100 ng/μL)
Hieff Tr...