In gene research, biopharma, and cell therapy, efficiency and purity are always key. Traditional endotoxin-free plasmid prep often means long workflows, complex steps, and residual endotoxin risks.
Now, the new Low-Endotoxin Plasmid Prep Kits change the game — delivering ultra-pure DNA in just 30 minutes, combining speed with purity.
- Plasmid Mini Kit: Quickly extract 20–50 µg of high-purity plasmid DNA from 1–10 mL E. coli LB culture. Residual endotoxin is extremely low (<0.1 EU/µg DNA).
- Plasmid Midi Kit: Extract 0.5–2 mg of high-copy plasmid DNA from 150–300 mL E. coli LB culture with yields up to 80–90%. Residual endotoxin remains ultra-low (<0.1 EU/µg DNA).
Fast, pure, and reliable — optimized plasmid DNA prep for transfection, viral packaging, and therapeutic research.
|
Feature |
Traditional Plasmid Prep |
Low-Endotoxin Plasmid Prep |
|
Endotoxin level |
Often >5 EU/µg |
Ultra-low, safe for sensitive cells |
|
Workflow |
Complex, time-consuming |
Streamlined, ~30 minutes |
|
Cell impact |
Can cause apoptosis, stress responses, false positives |
Reliable transfection and stable cell performance |
|
Applications |
General cloning |
Stem cell transfection, viral vector packaging, gene therapy |
Features
- Flexible Options: Choose Mini, Midi, or Maxi kits to match your workflow.
- Wide Sample Range: Supports small- to large-scale bacterial cultures (2–300 mL).
- High DNA Purity: Free from protein and RNA; ready for digestion, transformation, sequencing, and sensitive applications.
- High Yield: Mini kits up to 50 µg; Midi/Maxi kits up to 4 mg plasmid DNA.
- Ultra-Low Endotoxin: <0.01 EU/µg for mid-to-high copy plasmids, much lower than conventional methods.
- Easy Operation: Push-filter design simplifies handling; compatible with various rotor types.
Performance
1. Plasmid Miniprep Kit
(A) .High Yields
|
Kit |
Sample No. |
Concentration (ng/μL, Qubit) |
Concentration (ng/μL, Nanodrop) |
A260/280 |
A260/230 |
|
Supplier F* |
1 |
29.6 |
72.836 |
1.948 |
2.335 |
|
2 |
32.4 |
81.289 |
1.941 |
2.349 |
|
|
Yeasen-19001 |
3 |
74.8 |
184.149 |
1.938 |
2.335 |
|
4 |
78.2 |
171.941 |
1.925 |
2.318 |
|
|
Supplier M* |
5 |
69.6 |
141.349 |
1.877 |
2.32 |
|
6 |
52.8 |
134.178 |
1.882 |
2.325 |
Figure 1. Comparison of Plasmid DNA Yields from Equal Volumes of Bacterial Culture
Plasmid yield: 19001 > Supplier M* > Supplier F*.
(B).High Quality with Ultra‑low Endotoxin
Figure 2. Plasmid DNA extracted using Yeasen 19021, Supplier O*, and Supplier T* kits was transfected into HEK293 cells with pEGFP‑C1. Bright‑field and green‑fluorescence images were taken at 72 h post‑transfection, showing transfection efficiency in the order Yeasen 19021> Supplier T* > Supplier O*.
2. Plasmid Maxiprep Kit
(A). Higher Plasmid Yield and Purity
|
Kit |
Concentration (ng/µL) |
First Elution Volume (mL) |
Yield 1st Elution (µg) |
Yield 2nd Elution (µg) |
1st Elution Recovery Rate |
A260/280 |
A260/230 |
|
Supplier A* |
853.0 |
1.03 |
879 |
1068 |
82.30% |
1.81 |
2.29 |
|
Supplier A* |
631.6 |
1.00 |
630 |
739 |
85.24% |
1.81 |
2.26 |
|
19038ES |
942.9 |
1.07 |
1005 |
1189 |
84.53% |
1.84 |
2.41 |
|
19038ES |
928.8 |
1.06 |
983 |
1161 |
84.66% |
1.84 |
2.41 |
Result: The Hieff™ Pro Endo-free Plasmid Maxi Kit (19038ES) achieved 1.26× higher plasmid yield compared with Supplier A*, while maintaining comparable purity.
(B). Lower Endotoxin Residual
|
Kit |
Endotoxin Residual |
|
Supplier A* |
~0.1 EU/µg |
|
Supplier A* |
~0.1 EU/µg |
|
19037ES |
~0.5 EU/µg |
|
19037ES |
~0.5 EU/µg |
|
19038ES |
<0.01 EU/µg |
|
19038ES |
<0.01 EU/µg |
Result: The 19038ES kit consistently delivered plasmid DNA with endotoxin levels below 0.01 EU/µg, significantly lower than Supplier A*.
Customer Feedback
From 200 mL bacterial culture, up to 1.8 mg plasmid DNA was recovered with excellent purity and ultra-low endotoxin, fully meeting daily transfection needs.
User-friendly workflow – the push-column filter allows easy removal of bacterial debris with a single pull.
|
Kit |
Concentration (ng/µL) |
Yield (µg) |
A260/280 |
A260/230 |
Endotoxin Residual |
|
Supplier B* |
943.5 |
1415.3 |
1.83 |
2.21 |
~0.5 EU/µg |
|
Supplier B* |
852.5 |
1278.8 |
1.84 |
2.23 |
~0.5 EU/µg |
|
19038ES |
1221.4 |
1832.0 |
1.82 |
2.33 |
<0.1 EU/µg |
|
19038ES |
1264.1 |
1896.2 |
1.82 |
2.30 |
<0.1 EU/µg |
Result: Compared to Supplier B*, the 19038ES kit yielded higher DNA concentrations and lower endotoxin levels (<0.1 EU/µg vs ~0.5 EU/µg), ensuring superior plasmid quality for sensitive applications such as stem cell transfection and viral packaging.
Related Products
|
Category |
Name |
Cat. No. |
Size |
|
Plasmid miniprep |
19001ES50/ES70 |
50 T/200 T |
|
|
19021ES50/70 |
50 T/200 T |
||
|
Plasmid midiprep |
Hieff™ Endo-free Plasmid Mini-Midi Kit |
19022ES08/50/70 |
8 T/50 T/200 T |
|
19023ES02/20 |
2 T/20 T |
||
|
Plasmid maxiprep |
19037ES02/10 |
2 T/10 T |
|
|
19038ES02/10 |
2 T/10 T |
Citations & References
[1] Zhang C, Liu Y, Zhang T, Lv C, Zang J, Zhao G. Structural comparison between the DNA-protective ability of scallop and shrimp ferritin from iron-induced oxidative damage. Food Chem. 2022;386:132827. doi:10.1016/j.foodchem.2022.132827(IF:7.514)
[2] Hei H, Gao J, Dong J, et al. BK Knockout by TALEN-Mediated Gene Targeting in Osteoblasts: KCNMA1 Determines the Proliferation and Differentiation of Osteoblasts. Mol Cells. 2016;39(7):530-535. doi:10.14348/molcells.2016.0033(IF:2.670)
[3] Han P, Cao P, Yue J, et al. Knockdown of hnRNPA1 Promotes NSCLC Metastasis and EMT by Regulating Alternative Splicing of LAS1L exon 9. Front Oncol. 2022;12:837248. Published 2022 Jun 23. doi:10.3389/fonc.2022.837248(IF:5.738)
[4] Zhang J, Wang W, Feng N, Jiang X, Zhu S, Chen YQ. Ndufa6 regulates adipogenic differentiation via Scd1. Adipocyte. 2021;10(1):646-657. doi:10.1080/21623945.2021.2007590(IF:4.534)
[5] Zhu S, Zhang J, Wang W, Jiang X, Chen YQ. Blockage of NDUFB9-SCD1 pathway inhibits adipogenesis : Blockage of NDUFB9-SCD1 pathway inhibits adipogenesis. J Physiol Biochem. 2022;78(2):377-388. doi:10.1007/s13105-022-00876-7(IF:4.158)