Metagenomics is transforming the way we study microorganisms by enabling comprehensive profiling of microbial communities—shedding light on their diversity, functions, and interactions with hosts and the environment. First introduced in 1998, metagenomics has rapidly advanced thanks to high-throughput sequencing technologies. Today, metagenomic next-generation sequencing (mNGS) plays a pivotal role in clinical pathogen detection, with applications expanding across agriculture, food safety, environmental monitoring, and biotechnology.
At the heart of this expansion are innovations in library preparation. Yeasen’s Enzymatic DNA Fragmentation Library Prep Kits are designed to accelerate pathogen detection, delivering faster turnaround times, higher sensitivity, and cost-efficiency compared with traditional approaches.
Why Speed Matters in Pathogen Metagenomics
When dealing with infectious diseases, time is critical. Conventional pathogen detection methods—such as culture, PCR, or targeted assays—are often limited to known organisms, can be time-consuming, and may miss co-infections. By contrast, mNGS enables unbiased detection of both common and rare pathogens directly from clinical or environmental samples.
Yeasen’s enzymatic fragmentation DNA library prep kits help streamline this process. Unlike mechanical fragmentation methods, enzymatic fragmentation reduces workflow complexity, minimizes hands-on time, and provides more uniform coverage. This results in libraries that are not only prepared more quickly, but also with higher quality, enabling researchers and clinicians to obtain actionable results faster.
Clinical Applications
Metagenomic sequencing has already shown significant promise in the medical field:
- Infectious disease diagnostics: mNGS enables unbiased detection of bacteria, viruses, fungi, and parasites from respiratory, gastrointestinal, reproductive, and neurological samples.
- Emerging pathogen discovery: mNGS allows researchers to identify novel or unexpected pathogens that evade conventional methods.
- Public health surveillance: mNGS supports monitoring of multidrug-resistant organisms and outbreak tracking, enhancing response to public health threats.
Beyond the Clinic: Expanding Applications
- Agriculture and animal health: Metagenomics reveals how soil, crop, and animal-associated microbes impact health, disease, and productivity. It is being applied to crop disease surveillance, livestock infection monitoring, and feed efficiency optimization.
- Food safety: Metagenomics supports microbial source tracking, quality control, and contamination monitoring throughout the food production chain.
- Environmental and energy research: Applications include bioremediation, wastewater monitoring, and characterization of microbial roles in biogeochemical cycles.
Why Choose Yeasen’s Enzymatic Library Prep Kits?
- Fast: Rapid fragmentation and streamlined workflows reduce preparation time.
- Reliable: Uniform fragmentation ensures consistent and reproducible sequencing data.
- Cost-effective: Lower reagent consumption helps reduce sequencing costs.
- Versatile: Compatible with a wide range of sample types, including clinical, agricultural, and environmental specimens.
By removing bottlenecks in library preparation, Yeasen’s enzymatic kits enable researchers and clinicians to focus on what matters most: generating insights and making informed decisions.
1. Ultra-Fast Library Prep Workflow
2. Broad sample compatibility
Figure 1. Rapid DNA Library Preparation with Broad Sample Type Compatibility.Compatible with gDNA, cDNA, and cfDNA, delivering library yields >1 μg.
3. Excellent microbial detection
Figure 2. Libraries were prepared from 10 ng of ZymoBIOMICS Microbial DNA Standard (Cat# D6306) using the Flash DNA Library Kit (Cat#N210004). Libraries were pooled and sequenced on an Illumina MiSeq (2 ×150 bp). The detected microbial composition closely matched the expected proportions. Expected composition: Cryptococcus neoformans 2%,
Saccharomyces cerevisiae 2%, Bacillus subtilis 12%, Escherichia coli 12%, Enterococcus faecalis 12%, Lactobacillus fermentum 12%, Listeria monocytogenes 12%, Pseudomonas aeruginosa 12%, Staphylococcus aureus 12%, and Salmonella enterica 12%.
4. Low DNA Residue
5. Strong Stability
Figure 3. Stability test of the DNA Library Prep Kit.
The kit maintained stable performance after 4 weeks of storage at 4°C and remained unaffected after up to 30 freeze–thaw cycles, demonstrating excellent stability under common laboratory conditions.
Product list
|
Category |
Name |
Cat. No. |
Size |
|
DNA lib prep kit |
12316ES |
24T /96 T |
|
|
12972ES |
24T /96 T |
||
|
12927ES |
24T /96 T |
||
|
RNA lib prep kit |
12340ES |
24T /96 T |
|
|
12341ES |
24T /96 T |
||
|
12254ES |
24T /96 T |
||
|
Beads |
12601ES03/08/56 |
1 mL/5 mL/60 mL |
|
|
12602ES03/08/56 |
1 mL/5 mL/60 mL |
||
|
Adapter |
Hieff NGS™ Stubby UDI Primer Kit for Illumina, PE adapter plus, Set1-Set4(384 index) |
13327-12330ES01/02 |
96×1 T/96×2 T |
|
Hieff NGS™ Unique Dual Barcode Primer Kit for MGI™, Set1-Set4(384 index) |
13350-13353ES01/02 |
96×1 T/96×2 T |
|
|
Library Quantitation |
12642ES60/76 |
100 T/500 T |
|
|
12645ES60/76 |