Beschreibung
Product Properties
|
Alternative Names |
CLMF, CLMF2, IL-12A, IL-12B, IL12, IL12 p70, IMD28, IMD29, NFSK, NKSF1, NKSF2, P35, IL-12 subunit p35, IL12A, interleukin 12 |
|
Species |
Cynomolgus |
|
Expression Sequence |
Ile23-Ser328 (IL-12B) & Arg57-Ser253 (IL-12A) |
|
Accession |
G7P6S2(IL-12B)&XP_005546300.2(IL-12A) |
|
Tag |
C-His |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 34.76 kDa (IL-12B) and 23.64 kDa (IL-12A). Due to glycosylation, the protein migrates to 45-48 kDa (IL-12B) and 38-44 kDa (IL-12A) based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
ELISA Data: Immobilized Cynomolgus IL-12, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. |
|
Reconstitution Method |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Storage
Store at -25 to -15°C. Once received, the product is valid for one year. After resuspension, store at -85 to -65°C. The product is valid for three months.
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Anfrage
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FAQ
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