Beschreibung
Activin A is a member of the TGF-β family and exhibits a wide range of biological activities, including the regulation of cell proliferation and differentiation, as well as the promotion of neuronal survival. Elevated levels of Activin A in human colorectal cancer and postmenopausal women are associated with colorectal cancer and breast cancer, respectively. The biological activity of Activin A can be neutralized by inhibin and the diffusible TGF-β antagonist follistatin. Activin A binds to two forms of Activin Receptor Type I (ActR-I-A and ActR-I-B) and two forms of Type II receptors (ActR-II-A and ActR-II-B). Activins are homodimers or heterodimers of various β subunits. They are produced as precursor proteins with an N-terminal propeptide, which is cleaved to release the C-terminal bioactive ligand.
Product Information
|
Product Name |
Product Number |
Specification |
|
HiActiTM Recombinant Human Activin A Protein,His Tag |
91717ES10 |
10 μg |
|
91717ES50 |
50 μg |
|
|
91717ES72 |
250 μg |
Performance Parameters
|
Synonyms |
Activin A, activin AB alpha polypeptide, erythroid differentiation factor, Erythroid differentiation protein, FSH-releasing protein, inhibin beta A chain |
|
Species |
Human |
|
Sequence |
Gly311-Ser426 |
|
Accession |
P08476 |
|
Tag |
N-His |
|
Expression System |
E.coli |
|
Molecular Weight |
Theoretical MW is approximately 20.0 kDa |
|
Purity |
> 95% as determined by SDS-PAGE |
|
Endotoxin |
Less than 1.0 EU per μg by the LAL method |
|
Formulation |
20mM NaHCO3, pH8.3 |
|
Appearance |
Liquid |
|
Bioactivity |
Determined by cell assay, Activin A inhibits the proliferation of mouse plasmacytoma cells MPC-11, with an ED50 of approximately 4ng/ml |
Storage
Store at -25 ~ -15°C. Valid for 1 year from the date of receipt. Avoid repeated freeze-thaw cycles. Centrifuge before opening the vial. It is recommended to aliquot into single-use portions for each experiment.
Product Features
High activity, high purity, low endotoxin, high stability.
Application Examples
Figure 1. SDS-PAGE
Human Activin A on SDS-PAGE under reduced (R) condition. The purity is greater than 95%.
Figure 2. Cell Based Assay
Determined by cell-based assay, Activin A protein inhibits the proliferation of mouse plasmacytoma cells MPC-11, with an ED50 of approximately 4 ng/mL.
Documents:
Manuals
Zahlung und Sicherheit
Ihre Zahlungsinformationen werden sicher verarbeitet. Wir speichern weder Kreditkartendaten noch Zugriff auf Ihre Kreditkarteninformationen.
Sie können auch mögen
Anfrage
FAQ
Das Produkt ist nur für Forschungszwecke bestimmt und nicht für die therapeutische oder diagnostische Anwendung bei Menschen oder Tieren. Produkte und Inhalte sind durch Patente, Marken und Urheberrechte von
Für bestimmte Anwendungen sind möglicherweise zusätzliche geistige Eigentumsrechte Dritter erforderlich.