Beschreibung
Fibroblast growth factor-6 (FGF-6) is a cytokine belonging to the heparin-binding members of the FGF family. Members of this family exhibit broad mitogenic and cell survival activities and are involved in various biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. FGF-6 binds to and signals through the FGF receptors FGFR1 and FGFR4. It is also essential for normal muscle regeneration. In vivo, high concentrations of recombinant FGF-6 upregulate FGFR1 and downregulate FGFR4, respectively, as FGFR1 promotes proliferation while FGFR4 promotes muscle differentiation. Transfection of the FGF6 gene into mammalian cells has been shown to possess oncogenic transformation activity. Although FGF-6 serves a protective function against mechanical muscle injury, it is overexpressed in the developing heart and regulates osteoblasts and osteoclasts during bone remodeling.
Specifications
|
Cat.No. |
91351ES10 / 91351ES50 / 91351ES60 / 91351ES76 |
|
Size |
10 μg / 50 μg / 100 μg / 500 μg |
Components
|
Name |
91351ES10 |
91351ES50 |
91351ES60 |
91351ES76 |
|
HiActiTM Recombinant Human FGF-6 Protein, His Tag |
10 μg |
50 μg |
100 μg |
500 μg |
Performance parameters
|
Synonyms |
fibroblast growth factor 6, HBGF-6, HST-2, FGF6 |
|
Species |
Human |
|
Expression Sequence |
Gly41-Ile208 |
|
Accession |
P10767 |
|
Tag |
C-His |
|
Expression System |
E.coli |
|
Molecular Weight |
18-19 kDa(Reducing) |
|
Purity |
>95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
<1.0 EU per 1 μg of the protein by the LAL method. |
|
Formulation |
PBS, pH7.4 |
|
Appearance |
Lyophilized Powder |
|
Biological activity |
Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblasts. The EC50 for this effect is typically less than 5 ng/mL. |
|
Reconstitution Instructions |
Centrifuge the tube before opening to collect the contents at the bottom. Reconstitute with ultra-pure water to a concentration of 0.1–0.5 mg/mL. The solution can be further diluted according to subsequent experimental requirements. |
Storage
Store at -25 to -15°C. Valid for 1 year from receipt. After reconstitution, store at 2 to 8°C for up to 7 days, or at -85 to -65°C for up to 3 months. To avoid repeated freeze-thaw cycles, we recommend aliquoting the protein upon first use.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves, for your safety.
Documents
Safety Data Sheet
Manuals:
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