Beschreibung
Interleukin-30 (IL-30) is a chain of the heterodimeric cytokine interleukin-27 (IL-27), and is therefore also known as IL-27 p28, with a predicted molecular weight of 24.9 kDa. As the p28 subunit, IL-30 associates with EBI3 to form IL-27. Also referred to as IL-27 p28 or IL-27A, it is considered an alternative to IL-27. It was initially thought to be an IL-12-like cytokine capable of promoting Th1 immunity, as it induces the expression of T-bet and IL-12Rβ2 by activating STAT1 during Th1 differentiation.
Specifications
|
Cat.No. |
90295ES08 / 90295ES20 / 90295ES60 / 90295ES76 |
|
Size |
5 μg / 20 μg / 100 μg / 500 μg |
Components
|
Name |
90295ES08 |
90295ES20 |
90295ES60 |
90295ES76 |
|
HiActi™ Recombinant Mouse IL-30 Protein, His Tag |
5 μg |
20 μg |
100 μg |
500 μg |
Performance parameters
|
Synonyms |
interleukin 30, IL-27-p28 subunit, IL27A; IL-27A; IL27p28, IL30 |
|
Species |
Mouse |
|
Expression Sequence |
MFPRPPGRPQLSLQELRREFTVSLHLARKLLSEVRGQAHRFAESHLPGVNLYLLPLGEQLPDVSLTFQAWRRLSDPERLCFISTTLQPFHALLGGLGTQGRWTNMERMQLWAMRLDLRDLQRHLRFQVLAAGFNLPEEEEEEEEEEEEERKGLLPGALGSALQGPAQVSWPQLLSTYRLLHSLELVLSRAVRELLLLSKAGHSVWPLGFPTLSPQP with polyhistidine tag at the C-terminus. |
|
Accession |
Q8NEV9 |
|
Tag |
C-His |
|
Expression System |
E.coli |
|
Molecular Weight |
The protein has a calculated MW of 25.47 kDa. The protein migrates as 28 kDa under reducing condition |
|
Purity |
>98% as determined by SDS-PAGE. |
|
Endotoxin |
<0.1 EU per 1 μg of the protein by the LAL method. |
|
Formulation |
The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. |
|
Appearance |
Lyophilized Powder |
|
Reconstitution Instructions |
Briefly centrifuge the vial at 3000 rpm for 5 minutes before opening. It is recommended to reconstitute the lyophilized powder with sterile water to a concentration of no less than 100 μg/mL, and let it stand for at least 20 minutes to ensure complete dissolution. The reconstituted solution can be further diluted and aliquoted. When performing further dilution and aliquoting, a carrier protein (0.1% BSA, 10% FBS, or 5% HSA) must be added. For serum-free experimental requirements, a 5% trehalose solution can be used as an alternative carrier. |
Storage
Store at -25 to -15°C. Valid for 1 year from receipt. After reconstitution, store at 2 to 8°C for up to 7 days, or at -85 to -65°C for up to 3 months. To avoid repeated freeze-thaw cycles, we recommend aliquoting the protein upon first use.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves,for your safety.
Documents:
Safety Data Sheet
Manuals:
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