Beschreibung
Medium Salt Active UltraNuclease is a recombinant non-specific endonuclease derived from Vibrio cholerae. Designed for bioprocessing and biomanufacturing workflows, it efficiently degrades all forms of DNA and RNA (double-stranded, single-stranded, linear, circular, native, or denatured) under physiological salt conditions, making it widely applicable for nucleic acid removal in biological products.
This novel endonuclease is expressed and purified in Pichia pastoris following genetic engineering, allowing it to directly replace traditional nucleases without altering existing process workflows. It exhibits activity across a broad pH range, with optimal performance at salt concentrations of 125–250 mM. Due to its exceptional performance under physiological salt conditions, it can be added directly to cell culture media or harvest supernatants without the need for buffer adjustments. These features make Medium Salt Active UltraNuclease an ideal choice for the production of AAV and LV viral vectors, as well as recombinant proteins.
Supplied as a sterile liquid enzyme, it is a colorless, transparent solution stored in a buffer containing 25 mM Bis-Tris, 5 mM MgCl₂, 400 mM NaCl, 50% Glycerol, and 0.05% Tween-20 (pH 7.0).
Features
Simplified Workflow
Optimal activity at 125–250 mM salt, with no salt adjustment required.
Broad Compatibility
Compatible with a wide range of cell culture media.
High Stability
Validated through accelerated, transport, freeze–thaw, and real-time stability testing.
Regulatory-Ready Support
Extensive project experience to support regulatory submissions and audits.
Components
|
Components No. |
Name |
20160ES25 (25 KU) |
20160ES50 (50 KU) |
20160ES60 (100 KU) |
20160ES80 (1 MU) |
|
20160 |
UCF.ME™ Medium Salt Active UltraNuclease |
100 μL |
200 μL |
400 μL |
4 mL |
Specifications
|
Source |
Pichia pastoris |
|
Molecular Weight |
24.5 kDa |
|
Isoelectric Point (pI) |
8.42 |
|
Purity |
≥99% |
|
Enzyme Activity |
250–300 U/μL |
|
Optimum Temperature |
37°C (Working range: 20–50°C) |
|
Cofactor |
>0.5 mM Mg²⁺ |
|
Storage Buffer |
25 mM Bis-Tris, 5 mM MgCl₂, 400 mM NaCl, 50% Glycerol, 0.05% Tween-20, pH 7.0 |
|
Unit Definition |
One unit (U) is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 minutes at 37°C in a reaction mixture containing 25 mM Tris-HCl (pH 7.6 at 25°C), 2.5 mM MgCl₂, 150 mM NaCl, and 50 μg/mL calf thymus DNA. |
Storage
This product should be stored at -25~-15℃ for 2 years.
Figure
Optimal Nuclease Activity at Physiological Salt Concentrations (150–200 mM)
Figure 1. The mid-salt nuclease exhibits optimal enzymatic activity under physiological salt concentrations (150–200 mM), enabling direct use in cell culture supernatants without salt adjustment.
Documents:
Safety Data Sheet
Manuals
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