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This kit is designed for the isolation and purification of high-quality total RNA from a wide variety of sample types, including animal tissues (heart, liver, spleen, lung, muscle, fat, brain, etc.), cells, common plants, and polysaccharide‑ and polyphenol‑rich plant samples. A unique magnetic bead system combined with a carefully optimized buffer formulation efficiently captures the released nucleic acids, yielding RNA of high purity with minimal carry‑over of genomic DNA, protein and other impurities. The purified RNA is suitable for a broad range of downstream applications such as RT-PCR, real-time PCR, microarray analysis, Northern blot, dot blot, poly(A) selection, in vitro translation, RNase protection assay, and molecular cloning. The kit is fully compatible with YEASEN automated nucleic acid extraction instruments and the Thermo Scientific KingFisher Flex 96 and comparable systems. YEASEN offers integrated solutions for high‑throughput automated nucleic acid extraction.
Features
Broad sample compatibility: Works with various animal and plant tissues, including liver, kidney, spleen, heart, skin, aquatic samples, leaves, roots, fruits, and seeds.
Fast and simple: Extraction takes less than 1 hour per sample. Fully compatible with Yeasen 16/48/96-channel extraction instruments.
High-purity RNA: Includes DNase I for complete DNA removal. Ready for RT-PCR, library prep, and other downstream applications.
Components
|
Category |
No. |
Name |
18613ES48 |
|
Part I |
18613-A |
DNase I |
0.6 mL/tube × 1 |
|
Part II |
18613-B |
Proteinase K |
1.1 mL/tube × 1 |
|
Part III |
18613-C |
Lysis Buffer AL |
16 mL/bottle × 1 |
|
18613-D |
Lysis Buffer PL |
50 mL/bottle × 1 |
|
|
18613-E |
Digestion Buffer DR |
40 mL/bottle × 1 |
|
|
18613-F |
Binding Buffer RB |
2 mL/bottle × 1. Add 28 mL isopropanol prior to first‑time use |
|
|
18613-G |
Magnetic Bead Suspension |
1.1 mL/tube × 1 |
|
|
18613-H |
Wash Buffer DW1 |
19 mL/bottle × 1. Add 19 mL absolute ethanol prior to first‑time use |
|
|
18613-I |
Wash Buffer DW2 |
18 mL/bottle × 1. Add 72 mL absolute ethanol prior to first‑time use |
|
|
18613-J |
Elution Buffer RE |
5 mL/bottle × 1 |
Notes
1. If precipitation or turbidity appears in any reagent (common when stored at low ambient temperatures), warm to 30 °C and mix until fully dissolved.
2. Magnetic beads may carry over into the eluate. Avoid aspirating magnetic beads when transferring eluted RNA.
3. Avoid repeated freeze-thaw cycles of frozen samples, as this may compromise nucleic acid quality.
4. For your safety and health, wear a lab coat and disposable gloves when handling this product.
5. Several buffers contain guanidinium salts. Do NOT mix with oxidizing disinfectants such as sodium hypochlorite; toxic gas may form. Dispose of all kit‑related waste in accordance with local institutional regulations for biohazardous waste.
6. This product is for research use only.
Storage
FAQ
Citations
Documents
| Manual |
|---|
| 18613-Manual-Ver.EN20261026.pdf |
Applications
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Anfrage
FAQ
Das Produkt ist nur für Forschungszwecke bestimmt und nicht für die therapeutische oder diagnostische Anwendung bei Menschen oder Tieren. Produkte und Inhalte sind durch Patente, Marken und Urheberrechte von
Für bestimmte Anwendungen sind möglicherweise zusätzliche geistige Eigentumsrechte Dritter erforderlich.

