Beschreibung
FuniCutTM enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All FuniCutTM enzymes show superior activity in the universal FuniCutTM Buffer and are able to digest DNA in 5~15 minutes.This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. FuniCutTM enzymes have passed multiple strict quality control steps, and can be used to digest plasmid, genomic and viral DNA as well as PCR products.
Specifications
|
Enzyme |
Xbal |
|
Cutting Site |
5'-T↓CTAGA-3' 3'-AGATC↑T-5' |
|
Optimal Reaction Temperature |
37℃ |
|
Sensitive to Heat Inactivation |
YES |
|
Type lls RE |
NO |
|
Methylation Sensitivity |
Not CpG Methylation-Sensitive, Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive,Not EcoKl Methylation-Sensitive, Not EcoBI Methylation-Sensitive |
|
Star Activity |
3 h incubation did not show star activity, and delayed enzyme digestion might show star activity |
|
Isoschizomers |
NO |
Components
|
Components No. |
Name |
15033ES76 |
|
15033-A |
FuniCutTM Xbal |
500 μL |
|
15033-B |
10xFuniCutTM Buffer |
3 x 1 mL |
|
15033-C |
10xFuniCutTM Color Buffer* |
3 x 1 mL |
【Note】: 10× FuniCutTM Color Buffer includes red and yellow tracking dyes, allowing direct loading of reaction products onto agarose gels without additional loading buffer. In 1% agarose gel electrophoresis, the red dye co-migrates with ~2500 bp double-stranded DNA fragments, while the yellow dye migrates similarly to ~10 bp double-stranded DNA fragments.
Shipping and Storage
This product should be stored at -25℃ ~ -15℃ for up to 2 years.
Documents:
Safety Data Sheet
Manuals
15033_Manual_Ver.EN20250801.pdf
Zahlung und Sicherheit
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Anfrage
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FAQ
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