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The FuniCut™ series of restriction endonucleases are rapidly acting, genetically engineered enzymes capable of precisely cutting DNA within 5 to 15 minutes. They are ideal for the rapid digestion of plasmid DNA, PCR products, genomic DNA, and more. The FuniCut™ series shares a universal digestion buffer, simplifying the reaction setup, and offers excellent enzyme activity redundancy, making it easy to handle substrate excess or challenging templates.
Specifications
|
Enzyme |
KpnI |
|
Recognition Site |
5'-GGTAC↓C-3' 3'-C↑CATGG-5'
|
|
Recommended Reaction Conditions |
1× FuniCut™ Buffer; incubate at 37°C. |
|
Enzyme Activity |
100 U/μL |
|
Inactivation Conditions |
Incubate at 80°C for 20 minutes. |
Components
|
Components No. |
Name |
15027ES60 |
|
15015-A |
FuniCut™ KpnI |
200 μL |
|
15015-B |
10×FuniCut™ Buffer |
2x 1 mL |
|
15015-C |
10×FuniCut™ Color Buffer* |
2x 1 mL |
[Note]:*10xFuniCut™ Color Buffer includes red and yellow tracking dyes, allowing direct use of the product for gel electrophoresis. The red dye migrates similarly to a 2500 bp double-stranded DNA fragment in 1% agarose gel; the yellow dye migrates similarly to a 10 bp double-stranded DNA fragment in 1% agarose gel.
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