Bsu DNA polymerase (Large Fragment) is derived from Bacillus subtilis. This enzyme retains the 5′-to-3′ polymerase activity of Bsu DNA polymerase I but lacks the 5′-to-3′ exonuclease domain. The large fragment itself lacks 3′-to-5′ exonuclease activity. It is a DNA isothermal amplification polymerase with strand-displacement activity and is mainly used in Recombinase Polymerase Amplification (RPA).
Product Information
|
Catalog No. |
14415ES72 / 14415ES80 / 14415ES90 / 14415ES97 |
|
Size |
2,500 U / 5,000 U / 50,000 U / 250,000 U |
|
Unit Definition |
One unit (U) is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble material within 30 min at 37°C. |
|
Storage Buffer |
25 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C. |
|
Heat Inactivation |
75°C for 20 min. |
Component Information
|
Product Name |
14415ES72 |
14415ES80 |
14415ES90 |
14415ES97 |
|
Bsu DNA Polymerase (Large Fragment, 50 U/μL) |
50 μL |
100 μL |
1 mL |
5 × 1 mL |
Feature
- Due to the lack of 3′-to-5′ exonuclease activity, Bsu DNA polymerase (Large Fragment) cannot remove unpaired 3′ overhangs and is therefore not suitable for generating blunt ends.
- At 25°C, Bsu DNA polymerase (Large Fragment) retains 50% activity, which is twice that of Klenow Fragment (3′-to-5′ exo-) at the same temperature.
- Storage buffer: 25 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C.
- Heat inactivation: 75°C for 20 min.
Storage
Store at -25°C to -15°C. The shelf life is 2 years.
Documents:
Safety Data Sheet
Manuals:
Storage
FAQ
Citations
Documents
Applications
Sie können auch mögen
Anfrage
FAQ
Das Produkt ist nur für Forschungszwecke bestimmt und nicht für die therapeutische oder diagnostische Anwendung bei Menschen oder Tieren. Produkte und Inhalte sind durch Patente, Marken und Urheberrechte von
Für bestimmte Anwendungen sind möglicherweise zusätzliche geistige Eigentumsrechte Dritter erforderlich.